Phytochemical composition and in vitro pharmacological investigations of Neurada procumbens L. (Neuradaceae): A multidirectional approach for industrial products

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dc.contributor.authorKhurshid, Umair
dc.contributor.authorAhmad, Saeed
dc.contributor.authorSaleem, Hammad
dc.contributor.authorNawaz, Hafiz Awais
dc.contributor.authorZengin, Gökhan
dc.contributor.authorLocatelli, Marcello
dc.contributor.authorMahomoodally, Mohamad Fawzi
dc.contributor.authorZainal Abidin, Syafiq Asnawi
dc.contributor.authorTousif, Muhammad Imran
dc.contributor.authorAhemad, Nafees
dc.date.accessioned2020-03-26T20:19:09Z
dc.date.available2020-03-26T20:19:09Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThe plants of genus Neurada have been utilized for medicinal purposes. This study probed into the biological propensities and endeavored to achieve the detailed phytochemical profile, via ultra-high performance liquid chromatography -UHPLC-MS analysis and high performance liquid chromatography photodiode array -HPLC-PDA of different polarity extracts (methanol, n-hexane, chloroform, and n-butanol) of Nevada procumbens L. The biological features were studied by determining the antioxidant potential via 2,2-Diphenyl-1-picrylhydrazyl-DPPH, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid -ABTS, ferric reducing antioxidant power -FRAP, cupric reducing antioxidant power -CUPRAC, phoshomolybdenum and metal chelation assays and clinically significant major enzymes (cholinesterases, alpha-amylase, alpha-glucosidase and tyrosinase inhibition). Moreover, the correlation among the biological activities and total bioactive contents of the extracts were studied via multivariate statistical analysis. The methanol and n-butanol extracts revealed the presence of total phenolic and flavonoid contents in highest concentrations which tend to correlate with their maximum anti-oxidant capacities for radical scavenging and reducing power assays. The n-hexane extract was most active in the phosphomolybdenum assays whereas the chloroform extracts showed the highest metal chelation potential. The UHPLC-MS phytochemical profiling of the methanolic extract (both positive and negative ionization mode) revealed the existence of eighteen phytochemicals representing four different classes (phenolics, flavonoids, sesquiterpenoids, and alkaloids). The n-butanol extract inhibited acetylcholinesterase (4.10 mg galantamine equivalent -GALAE/g) and tyrosinase (127.65 mg kojic acid equivalent -KAE/g) significantly as compared to the control. The n-hexane extract exhibited highest inhibition against butyrylcholinesterase (1.93 mg GALAE/g) and alpha-glucosidase (59.0 mmol acarbose equivalent -ACAE/g), while chloroform extract exhibited prominent inhibitory action inimical to alpha-amylase (0.65 mmol ACAE/g). Overall, it was observed that FRAP and tyrosinase activities were the most contributive biological activities for the formation of first component with p values of 0.018 and 0.023, respectively, whereas, for the formation of second component, glucosidase activity was the most contributive assay with p value of 0.002. It can be concluded that the manifestation of dynamic phytochemicals with multi-pharmacological potential marks N. procumbens as a prospective origin of health-promoting molecules warrants further exploration as a unique therapeutic medicinal plant.en_US
dc.identifier.citationKhurshid, U., Ahmad, S., Saleem, H., Nawaz, H. A., Zengin, G., Locatelli, M., Mahomoodally, M. F., Zainal Abidin, S. A., Tousif, M. I., Ahemad, N. (2019). Phytochemical Composition and in vitro Pharmacological Investigations of Neurada procumbens L. (Neuradaceae): A Multidirectional Approach for Industrial Products. Industrial Crops and Products, 142, 111861.
dc.identifier.doi10.1016/j.indcrop.2019.111861en_US
dc.identifier.issn0926-6690en_US
dc.identifier.issn1872-633Xen_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.indcrop.2019.111861
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38108
dc.identifier.volume142en_US
dc.identifier.wosWOS:000499762200024en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorZengin, Gökhan
dc.language.isoenen_US
dc.publisherELSEVIERen_US
dc.relation.ispartofINDUSTRIAL CROPS AND PRODUCTSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectUHPLC-MSen_US
dc.subjectExtractionen_US
dc.subjectEnzyme inhibitionen_US
dc.subjectAntioxidanten_US
dc.subjectBioactive compoundsen_US
dc.titlePhytochemical composition and in vitro pharmacological investigations of Neurada procumbens L. (Neuradaceae): A multidirectional approach for industrial productsen_US
dc.typeArticleen_US

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