Evaluation of hydrogen production by Rhodobacter sphaeroides OU001 and its hupSL deficient mutant using acetate and malate as carbon sources

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dc.contributor.authorKars, Goekhan
dc.contributor.authorGunduz, Ufuk
dc.contributor.authorYucel, Meral
dc.contributor.authorRakhely, Gabor
dc.contributor.authorKovacs, Kornel L.
dc.contributor.authorEroglu, Inci
dc.date.accessioned2020-03-26T17:38:37Z
dc.date.available2020-03-26T17:38:37Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractRhodobacter sphaeroides O.U.001 is one of the candidates for photobiological hydrogen production among purple non-sulfur bacteria. Hydrogen is produced by Mo-nitrogenase from organic acids such as malate or lactate. A hupSL in frame deletion mutant strain was constructed without using any antibiotic resistance gene. The hydrogen production potential of the R. sphaeroides O.U.001 and its newly constructed hupSL deleted mutant strain in acetate media was evaluated and compared with malate containing media. The hupSL(-) R. sphaeroides produced 2.42 l H-2/l culture and 0.25 l H-2/l culture in 15 mM malate and 30 mM acetate containing media, respectively, as compared to the wild type cells which evolved 1.97 l H-2/l culture and 0.21 l H-2/l culture in malate and acetate containing media, correspondingly. According to the results, hupSL- R. sphaeroides is a better hydrogen producer but acetate alone does not seem to be an efficient carbon source for photo-heterotrophic H-2 production by R. sphaeroides. (C) 2009 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipMiddle East Technical University ResearchMiddle East Technical University [BAP-08-11-DPT2002K120510-BTEK-6]; Biological Research Center of Hungarian Academy of Sciences; EU 6th FPEuropean Union (EU) [HYVOLUTION-019825]; TOBITAK-BIDEBTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThis study has been supported by Middle East Technical University Research fund with the project number: BAP-08-11-DPT2002K120510-BTEK-6, Biological Research Center of Hungarian Academy of Sciences (The ITC Program), the EU 6th FP project "HYVOLUTION-019825" and the support from TOBITAK-BIDEB is also acknowledged gratefully.en_US
dc.identifier.doi10.1016/j.ijhydene.2009.01.016en_US
dc.identifier.endpage2190en_US
dc.identifier.issn0360-3199en_US
dc.identifier.issn1879-3487en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage2184en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.ijhydene.2009.01.016
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23531
dc.identifier.volume34en_US
dc.identifier.wosWOS:000264532600013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF HYDROGEN ENERGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectRhodobacter sphaeroidesen_US
dc.subjectBiohydrogenen_US
dc.subjectUptake hydrogenaseen_US
dc.subjectAcetateen_US
dc.subjectMalateen_US
dc.titleEvaluation of hydrogen production by Rhodobacter sphaeroides OU001 and its hupSL deficient mutant using acetate and malate as carbon sourcesen_US
dc.typeArticleen_US

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