Comparison of two different primer sets for detection of Pasteurella caballi in bronchoalveolar lavage fluids samples from thoroughbred Arabian foals, using PCR

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dc.contributor.authorSayin, Zafer
dc.contributor.authorErganis, Osman
dc.contributor.authorSakmanoglu, Asli
dc.contributor.authorHadimli, Hasan H.
dc.contributor.authorPinarkara, Yasemin
dc.contributor.authorMaden, Mehmet
dc.contributor.authorAl Shattrawi, Huda J. G.
dc.date.accessioned2020-03-26T19:23:26Z
dc.date.available2020-03-26T19:23:26Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn the present study, Pasteurella caballi (P. caballi) was isolated and identified in bronchoalveolar lavage fluid and lung samples from thoroughbred Arabian foals using conventional microbiological methods. Subsequently, the ability of two different PCR primer sets was evaluated for detection and confirmation of P. caballi. Primer sets 1 and 2, targeting the 16S rRNA gene of P. caballi, were designed using the Primer 3 and Primer-BLAST programs, respectively. PCR was performed to confirm P. caballi strains and to detect it directly in the bronchoalveolar lavage fluid and lung samples. In total, 35 Pasteurella spp. were isolated from 25 (38.4 %) of 65 bronchoalveolar lavage fluid samples, and 10 (58.8 %) of 17 lung samples. These strains were identified as P. caballi based on conventional microbiological and biochemical characteristics. The sensitivities of primers 1 and 2 were determined lobe 100 % to confirm cultured P. caballi strains. However, the specificity of P. caballi detection was lower with primer set-1 than primer set-2 in bronchoalveolar lavage fluid and lung samples. The sensitivity and specificity of primer set-2 were confirmed by gene sequence analysis. This study indicates that the 16S rRNA-PCR method, using primer set-2, provides a rapid and accurate tool for the detection and confirmation of P. caballi isolates in bronchoalveolar lavage fluid and lung samples from foals.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108G030]en_US
dc.description.sponsorshipThis research was supported by the Scientific and Technological Research Council of Turkey (Project number. 108G030). We thanks to Dr. Eray Atil (Istanbul Pcndik Veteriner Kontrol Enstitusu) for gene sequence analaysis.en_US
dc.identifier.endpage181en_US
dc.identifier.issn0372-5480en_US
dc.identifier.issn1331-8055en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage173en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33389
dc.identifier.volume86en_US
dc.identifier.wosWOS:000379525700002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUNIV ZAGREB VET FACULTYen_US
dc.relation.ispartofVETERINARSKI ARHIVen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectArabian foalsen_US
dc.subjectPasteurella caballien_US
dc.subjectpolymerase chain reactionen_US
dc.subjectprimer-BLASTen_US
dc.subjectprimer 3en_US
dc.subject16S rRNAen_US
dc.titleComparison of two different primer sets for detection of Pasteurella caballi in bronchoalveolar lavage fluids samples from thoroughbred Arabian foals, using PCRen_US
dc.typeArticleen_US

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