Buzağılarda Solunum Yolu Enfeksiyonlarının Tanısında Bronkoalveolar Lavaj Sıvısı Muayenelerinin Önem
Yükleniyor...
Dosyalar
Tarih
2002
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Yapılan çalışmada buzağılanın solunum sistemi enfeksiyonlarının tanısında BAL sıvısındaki biyokimyasal ve sitolojik değişikliklerin belirlenmesinin yaranı araştırıldı.Bu amaçla, 18 akut, 17 kronik solunum sistemi enfeksiyonlu ve 11 sağlıklı buzağı kullanıldı. Tüm buzağıların klinik muayenesi, tam kan analizi ve BAL sivisi analizleri yapıldı. Bron- koalveolar lavaj sıvısı sitolojik, biyokimyasal ve bakteriyolojik yönden incelendi. Bronkoalveolar lavaj sıvisi laktat de- hidrogenaz (LDH), alkalen fosfataz (ALP), aktiviteleri ve total protein konsantrasyonları, akut ve kronik solunum enfeksiyonu bulunan buzağılarda, sağlıklı gruba göre önemli ölçüde (p<0.01) yüksek olarak belirlendi. Sağlıklı grup ile karşılaştırıldığında akut ve kronik solunum enfeksiyonu bulunan buzağılanın BAL sıvısı nötrofil oranları önemli (p<0.01) ölçüde yüksek bulundu. Bronkoalveolar lavaj sıvisi makrofaj oranı akut ve kronik solunum sistemi enfeksiyonu bulunan buzağılarda normal buzağılara göre düşüktü. Kronik solunum sistemi enfeksiyonu bulunan buzağıların BAL sıvısı makrofaj oranlan akut enfeksiyonlu gruba göre daha yüksekti. Bu çalışmanın sonuçlanı BAL SIVISI analizlerinin pnömonik buzağıların spesifik tanısında hızlı, basit ve güvenli bir yöntem olduğuna karar verildi. Diagnostik amaçla BAL Svisi LDH, ALP aktiviteleri ve makrofaj, nötrofil yüzdelerinin belirlenmesi değerli bulunmuştur. Solunum sisteminin sitolojisi, daha kompleks diagnostik işlemler yapılana kadar hastaya uygulanacak işlemlerin seçilmesinde yönelik bilgi ve etiyopatolojik bir tanı sağlayabilir.
The present investigation evaluated the changes of cytological constitutents in bronchoalveolar lavage (BAL) fluid and to detect utility for diagnosis of respiratory disease in calves. For this purpose, 18 calves with acute respiratory disease, 17 calves with chronic respiratory disease and 11 healthy calves were used as materials. All cal- ves were examined with regard to clinical examination, complete blood cell counts and BAL fluid analysis. BAL fluid was investigated cytologically, biochemically and bacteriologically. BAL fluid lactate dehydrogenase (LDH), alkaline phosphatase (ALP) activities and total protein (TP) concentrations were significantly higher (p< 0.01) in the calves with acute and chronic respiratory infection compared to the normal calves. BAL fluid neutrophil percentages were significantly higher (p< 0.01) in the calves with acute and chronic respiratory infection compared to the normal calves. Macrophage percentages in BAL fluid were significantly decreased in the calves with acute and chronic respiratory in- fection. In the same time, BAL fluid macrophage percentage was significantly higher in the calves with chronic res- piratory infection compared to that of the calves with acute respiratory infection. The results of this study showed that, transtracheal BAL fluid analysis was a fast, safe, simple technique producing rapid diagnosis in pneumonic calves. The diagnostic potential of the percentage of alveolar macrophage and neutrophil counts in BAL fluid were validated. Cytologic examination of the respiratory tract may provide a etiopathologic diagnosis or information for improved case management, until more complex diagnostic procedures are performed.
The present investigation evaluated the changes of cytological constitutents in bronchoalveolar lavage (BAL) fluid and to detect utility for diagnosis of respiratory disease in calves. For this purpose, 18 calves with acute respiratory disease, 17 calves with chronic respiratory disease and 11 healthy calves were used as materials. All cal- ves were examined with regard to clinical examination, complete blood cell counts and BAL fluid analysis. BAL fluid was investigated cytologically, biochemically and bacteriologically. BAL fluid lactate dehydrogenase (LDH), alkaline phosphatase (ALP) activities and total protein (TP) concentrations were significantly higher (p< 0.01) in the calves with acute and chronic respiratory infection compared to the normal calves. BAL fluid neutrophil percentages were significantly higher (p< 0.01) in the calves with acute and chronic respiratory infection compared to the normal calves. Macrophage percentages in BAL fluid were significantly decreased in the calves with acute and chronic respiratory in- fection. In the same time, BAL fluid macrophage percentage was significantly higher in the calves with chronic res- piratory infection compared to that of the calves with acute respiratory infection. The results of this study showed that, transtracheal BAL fluid analysis was a fast, safe, simple technique producing rapid diagnosis in pneumonic calves. The diagnostic potential of the percentage of alveolar macrophage and neutrophil counts in BAL fluid were validated. Cytologic examination of the respiratory tract may provide a etiopathologic diagnosis or information for improved case management, until more complex diagnostic procedures are performed.
Açıklama
Anahtar Kelimeler
Buzağı, Solunum Sistemi Hastalıkları, Bronkoalveolar Lavaj, BAL, Sitoloji, ALP, LDH, Call, Respiratory Disease, Bronchoalveolar Lavage Fluid
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
18
Sayı
1-2
Künye
Birdane, F. M., Aslan, V., (2002). Buzağılarda Solunum Yolu Enfeksiyonlarının Tanısında Bronkoalveolar Lavaj Sıvısı Muayenelerinin Önem. Eurasian Journal of Veterinary Sciences, 18(1-2), 41-51.
