A new HPLC method for simultaneous analysis of sterols, tocopherols, tocotrienols, and squalene in olive oil deodorizer distillates using a monolithic column with chemometric techniques

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dc.contributor.authorTarhan, Ismail
dc.contributor.authorKara, Huseyin
dc.date.accessioned2020-03-26T20:12:20Z
dc.date.available2020-03-26T20:12:20Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn this study, a simple, rapid, and special method was developed for the simultaneous quantification of sterols (campesterol, beta-sitosterol, and stigmasterol), tocotrienols (alpha, (beta + gamma), and delta), tocopherols (alpha, (beta + gamma), and delta), and squalene in an olive oil deodorizer distillate using monolithic-chromatographic systems with chemometric techniques. The chromatographic conditions which are the mobile phase polarity (P '), the flow rate (mL min(-1)) and the temperature of the column compartment (degrees C) were optimized by using three experimental calibration designs. The optimal chromatographic conditions obtained using a response surface methodology were as follows: the polarity of the mobile phase, 7.00, 6.00, 5.70, and 5.10 for sterols, tocotrienols, tocopherols, and squalene, respectively; the flow rate, 2.50 mL min(-1); the temperature of the column compartment, 38.41 degrees C; and detection, at 202 nm using a diode array detector. For calibrations of all of the bioactive compounds, correlation coefficient values (R-2) were obtained at high values close to one and the limit of detection and the limit of quantification were satisfactory. The monolithic column used had a low column pressure despite the high polarity and the high flow rate values of the mobile phases thanks to its porous structure and it made possible an efficient chromatographic separation.en_US
dc.description.sponsorshipScientific Research Projects Foundation of Selcuk UniversitySelcuk University [16101011]en_US
dc.description.sponsorshipThe authors of this study thank the Scientific Research Projects Foundation of Selcuk University (SUBAP-grant number 16101011) for the financial support of this work produced from a part of Ismail Tarhan's PhD thesis.en_US
dc.identifier.doi10.1039/c9ay01525fen_US
dc.identifier.endpage4692en_US
dc.identifier.issn1759-9660en_US
dc.identifier.issn1759-9679en_US
dc.identifier.issue36en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage4681en_US
dc.identifier.urihttps://dx.doi.org/10.1039/c9ay01525f
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37421
dc.identifier.volume11en_US
dc.identifier.wosWOS:000487048000016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherROYAL SOC CHEMISTRYen_US
dc.relation.ispartofANALYTICAL METHODSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleA new HPLC method for simultaneous analysis of sterols, tocopherols, tocotrienols, and squalene in olive oil deodorizer distillates using a monolithic column with chemometric techniquesen_US
dc.typeArticleen_US

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