THE EFFECTS OF ANTI-MITOTIC AGENTS ON DIHAPLOIDIZATION AND FERTILITY IN WINTER SQUASH (Cucurbita maxima Duch.) AND PUMPKIN (Cucurbita moschata Duch.) ANDROGENIC HAPLOIDS

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dc.contributor.authorKurtar, Ertan Sait
dc.date.accessioned2020-03-26T19:56:09Z
dc.date.available2020-03-26T19:56:09Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractDouble-haploidization (DH) is one of the favorable techniques to obtain 100% pure double haploid plants (DH's) for generating the new F-1 cultivars in a short time. The fecundity of this technique depends on the high quantity of haploids and also fertile DH's. However, there are no comprehensive reports on the chromosome doubling and fertility (fruit and seed-set) of winter squash and pumpkin haploids, currently. Thus, to obtain high frequency and fertile DH's, the efficiency of different anti-mitotic agents (colchicine, amiprophos methyl, trifluralin and oryzalin) was tested at various concentrations and exposure times for both in vitro and in vivo conditions. Haploid plantlets recovered from anther cultures of winter squash and pumpkin lines were used for DH program. The haploid plants were wholly immersed in aqueous solutions of antimitotic agents in vitro and apical parts of haploid plantlets were treated with anti-mitotic agents three times in vivo. Since some plants remained haploid, and the highest DH efficiency was obtained from multiple treatments of colchicine to shoot tips of haploid plants in the rate of 93.3%. In vivo multiple treatments of 1% colchicine for an hour was found to be the best doubling procedure for the recovery of high-frequency fertile DH's in our winter squash and pumpkin breeding program.en_US
dc.identifier.doi10.24326/asphc.2018.5.1en_US
dc.identifier.endpage14en_US
dc.identifier.issn1644-0692en_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage3en_US
dc.identifier.urihttps://dx.doi.org/10.24326/asphc.2018.5.1
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37021
dc.identifier.volume17en_US
dc.identifier.wosWOS:000448045200001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWYDAWNICTWO AKAD ROLNICZEJ W LUBLINIEen_US
dc.relation.ispartofACTA SCIENTIARUM POLONORUM-HORTORUM CULTUSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectdihaploidizationen_US
dc.subjectanti-mitotic agentsen_US
dc.subjectfertilityen_US
dc.subjectpumpkinen_US
dc.subjectwinter squashen_US
dc.titleTHE EFFECTS OF ANTI-MITOTIC AGENTS ON DIHAPLOIDIZATION AND FERTILITY IN WINTER SQUASH (Cucurbita maxima Duch.) AND PUMPKIN (Cucurbita moschata Duch.) ANDROGENIC HAPLOIDSen_US
dc.typeArticleen_US

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