Levobupivakainin ve ropivakainin izole insan umblikal arter ve veni üzerine in vitro vazoaktif etkileri
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Date
2011
Journal Title
Journal ISSN
Volume Title
Publisher
AVES YAYINCILIK
Access Rights
info:eu-repo/semantics/openAccess
Abstract
Amaç: Levobupivakain ve ropivakainin endotelli ve endotelsiz insan umblikal arteri ve umblikal veni üzerindeki in vitro vazoaktif etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Damar şeritleri, kasılma cevapları izometrik olarak poligrafa yazdırılmak üzere 37°C’de oksijen içinde %5 CO2 karışımı ile sürekli olarak gazlandırılan organ banyosu içine yerleştirildi. Endotelin etkisini değerlendirmek amacıyla dokuların bir bölümünün endotelleri mekanik olarak sıyrıldı. Seratonin (10-6 M 5-HT, n=7) ile önceden kasılma sağlandı. Maksimum kasılma cevapları elde edildikten sonra dokular yıkanarak dinlendirildi. Kümülatif tarzda levobupivakain (10-9-10-4 M; n=7) ve ropivakain (10-9-10-4 M; n=7) ilave edilerek konsantrasyon-cevap eğrileri elde edildi. Kasılma cevapları 5-HT ile alınan maksimum kasılma cevabının yüzdesi (%) şeklinde değerlendirildi. Bulgular: Levobupivakain ve ropivakainin umblikal arter ve ven düz kasında konsantrasyon bağımlı kontraksiyon meydana getirdikleri gözlemlendi. Levobupivakain ile maksimum kasılma (Emax) cevapları hem umblikal arter (79.2±2.5) hemde umblikal vende (71.1±2.6) ropivakaine göre (68.4±2 ve 36.2±2.8) anlamlı olarak daha yüksekti. Ajanların Emax cevapları umblikal vene göre umblikal arterde anlamlı olarak daha yüksekti . Endotelli ve endotelsiz dokular arasındaki kasılma cevaplarında istatistiksel olarak anlamlı fark belirlenmedi. Sonuç: Yüksek doz levobupivakain ve ropivakain, umblikal damarlarda gelişebilecek kontraksiyona bağlı olarak fetal kan akımını azaltabileceği sonucuna varılabilir.
Objective: In this in vitro study, we investigated the vasoactive effects of levobupivacaine and ropivacaine on vascular smooth muscle derived from human umbilical arteries and veins. Material and Methods: The strips were mounted in tissue baths at 37 degrees C continuously gassed with 5% CO2 in oxygen for isometric recording of contractile activity on a polygraph. The endothelium of some tissues was mechanically removed to assess the influence of the endothelium on contractility. The strips were precontracted with serotonin (10(-6) M 5-HT; n=7). After obtaining the maximal contraction, responses obtained by adding levobupivacaine and ropivacaine (10(-9)-10(-4) M; n=7) cumulatively were recorded. Contractions were expressed as the (% of 5HT maximal response) percentage of 5 HT's maximal response. Results: Both levobupivacaine and ropivacaine induce a concentration-dependent contraction in the smooth muscle cells of umbilical arteries and veins. Maximum contractile response (E-max) of levobupivacaine (79.2 +/- 2.5, 71.1 +/- 2.6) was higher than ropivacaine (68.4 +/- 2, 36.2 +/- 2.8) on both umbilical arteries and veins. There were no statistically significant differences between contraction responses of endothelium-intact and endothelium-denuded tissues. Conclusion: The results suggest that, in high concentrations, levobupivacaine and ropivacaine may affect umbilical blood flow by contracting the umbilical artery and vein, thus reducing fetal blood flow.
Objective: In this in vitro study, we investigated the vasoactive effects of levobupivacaine and ropivacaine on vascular smooth muscle derived from human umbilical arteries and veins. Material and Methods: The strips were mounted in tissue baths at 37 degrees C continuously gassed with 5% CO2 in oxygen for isometric recording of contractile activity on a polygraph. The endothelium of some tissues was mechanically removed to assess the influence of the endothelium on contractility. The strips were precontracted with serotonin (10(-6) M 5-HT; n=7). After obtaining the maximal contraction, responses obtained by adding levobupivacaine and ropivacaine (10(-9)-10(-4) M; n=7) cumulatively were recorded. Contractions were expressed as the (% of 5HT maximal response) percentage of 5 HT's maximal response. Results: Both levobupivacaine and ropivacaine induce a concentration-dependent contraction in the smooth muscle cells of umbilical arteries and veins. Maximum contractile response (E-max) of levobupivacaine (79.2 +/- 2.5, 71.1 +/- 2.6) was higher than ropivacaine (68.4 +/- 2, 36.2 +/- 2.8) on both umbilical arteries and veins. There were no statistically significant differences between contraction responses of endothelium-intact and endothelium-denuded tissues. Conclusion: The results suggest that, in high concentrations, levobupivacaine and ropivacaine may affect umbilical blood flow by contracting the umbilical artery and vein, thus reducing fetal blood flow.
Description
Keywords
Levobupivakain, ropivakain, vazokonstriksiyon, Levobupivacaine, ropivacaine, vasoconstriction
Journal or Series
Balkan Medical Journal
WoS Q Value
N/A
Scopus Q Value
Q3
Volume
28
Issue
2
Citation
Kılıçaslan, A., Duman, A., Şahin, A. S. (2011). Levobupivakainin ve Ropivakainin İzole İnsan Umblikal Arter ve Veni Üzerine İn Vitro Vazoaktif Etkileri. Balkan Medical Journal, 28(2), 164-168.
