Matrix metalloproteinases 2 and 9 activity after intra-articular injection of autologous platelet-rich plasma for the treatment of osteoarthritis in dogs

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dc.contributor.authorArican, Mustafa
dc.contributor.authorSimsek, Atilla
dc.contributor.authorParlak, Kurtulus
dc.contributor.authorAtli, Kamil
dc.contributor.authorSonmez, Gonca
dc.date.accessioned2020-03-26T19:54:37Z
dc.date.available2020-03-26T19:54:37Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIntra-articular injection of platelet-rich plasma offers a promising potential for treatment of osteoarthritis in dogs. Twenty dogs weighing 25 to 50 kg (mean 38 kg) with unilateral stifle osteoarthritis were used for the study. Fourteen dogs were given intra-articular platelet rich plasma treatment and 6 dogs were used as controls. Double centrifuge method was used to obtain platelet-rich plasma. Radiography and ultrasonography of the affected joint were carried out and scores for lameness severity and pain severity were assigned by the attending clinicians. Synovial fluid was collected under sterile conditions at pre-treatment and on the 1st, 3rd, 5th, 7th, 15th days, and 4, 8 and 12 weeks after treatment. Gelatin zymography and Enzyme-linked Immunosorbent Assay were used to determine the synovial fluid levels of matrix metalloproteinase-2 (MMP-2) and metalloproteinase-9 (MMP-9). No adverse effects associated with the injection of the platelet concentrate or saline were observed. Hudson Visual Analog Scale and Canine Brief Pain Inventory scores for all components were non-significantly different between weeks 0, 4, 12 for control dogs. Matrix metalloproteinase-9 was totally and MMP-2 was partially inhibited in the platelet-rich plasma group. In the control group, MMP-9 was partially inhibited during the first month and activation started later. Matrix metalloproteinase-2 was constant in control samples throughout the experimental period. Platelet-rich plasma is a safe and effective method for treatment of dogs with osteoarthritis, possibly more useful for early cases with mild and moderate osteoarthritis. It is suggested that plasma rich platelet should be injected several times at regular intervals instead of a single application.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2130175]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, Project no. 2130175).en_US
dc.identifier.doi10.2754/avb201887020127en_US
dc.identifier.endpage+en_US
dc.identifier.issn0001-7213en_US
dc.identifier.issn1801-7576en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage127en_US
dc.identifier.urihttps://dx.doi.org/10.2754/avb201887020127
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36766
dc.identifier.volume87en_US
dc.identifier.wosWOS:000438898800005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherVETERINARNI A FARMACEUTICKA UNIVERZITA BRNOen_US
dc.relation.ispartofACTA VETERINARIA BRNOen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectGrowth factoren_US
dc.subjectgelatinaseen_US
dc.subjectpain scoresen_US
dc.subjectlamenessen_US
dc.subjectanti-inflammatory effecten_US
dc.titleMatrix metalloproteinases 2 and 9 activity after intra-articular injection of autologous platelet-rich plasma for the treatment of osteoarthritis in dogsen_US
dc.typeArticleen_US

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