Analysis of promoter methylation of Dickkopf1 (DKK1) gene in breast cancer

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dc.contributor.authorAcar, Muradiye
dc.contributor.authorCora, Tulin
dc.contributor.authorToy, Hatice
dc.contributor.authorAcar, Hasan
dc.date.accessioned2020-03-26T18:23:51Z
dc.date.available2020-03-26T18:23:51Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractAim: Breast cancer is the most common malignancy in women worldwide. The Dickkopf1 (DKK1) gene product is a potent inhibitor of the Wnt pathway. DKK1 methylation status and its protein expression were examined in normal and cancer tissues of breast cancer patients. Materials and methods: We examined methylation changes in 83 tumor and adjacent normal tissues, and also in 9 normal breast tissues from unaffected women (no breast cancer history) as controls. DKK1 promoter methylation was assessed by methylation-specific polymerase chain reaction. Immunohistochemical staining was used to investigate DKK1 protein expression in the 9 controls and in 70 out of the 83 tumors as well as their adjacent normal tissues. Immunohistochemical results were categorized as positive or negative for DKK1. Results: DKK1 gene methylation was detected in 3 out of the 9 (33%) normal breast tissue samples of unaffected women. The DKK1 gene was hypermethylated in 58 out of the 83 (70%) tumor samples and 51 out of the 83 (61%) adjacent normal tissue samples. In immunohistochemistry, 33% of normal breast tissue from unaffected women, 31% of the tumors, and 17% of the adjacent normal tissues in the breast cancer patients were found to be DKK1-positive. DKK1 promoter methylation and protein expression were not associated with age, tumor size, lymph node status, histological grade, estrogen/progesterone receptor status, or HER2 positivity. Conclusion: Our results indicate that there is a concordant DKK1 methylation change between normal and cancerous breast tissue.en_US
dc.identifier.doi10.3906/sag-1204-30en_US
dc.identifier.endpage1387en_US
dc.identifier.issn1300-0144en_US
dc.identifier.issn1303-6165en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1379en_US
dc.identifier.urihttps://dx.doi.org/10.3906/sag-1204-30
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27740
dc.identifier.volume42en_US
dc.identifier.wosWOS:000321226500004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEYen_US
dc.relation.ispartofTURKISH JOURNAL OF MEDICAL SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectBreast canceren_US
dc.subjectDickkopf1en_US
dc.subjectDNA methylationen_US
dc.titleAnalysis of promoter methylation of Dickkopf1 (DKK1) gene in breast canceren_US
dc.typeArticleen_US

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