Comparison of Er,Cr:YSGG Laser and Hand Instrumentation on the Attachment of Periodontal Ligament Fibroblasts to Periodontally Diseased Root Surfaces: An In Vitro Study

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dc.contributor.authorHakkı, Sema S.
dc.contributor.authorKorkusuz, Petek
dc.contributor.authorBerk, Gizem
dc.contributor.authorDündar, Niyazi
dc.contributor.authorSağlam, Mehmet
dc.contributor.authorBozkurt, Buket
dc.contributor.authorPurali, Nuhan
dc.date.accessioned2020-03-26T17:47:34Z
dc.date.available2020-03-26T17:47:34Z
dc.date.issued2010
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: This study investigates the effects of erbium, chromium: yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser irradiation and hand instrumentation on the attachment of periodontal ligament (PDL) fibroblasts to periodontally involved root surfaces. Methods: Twenty-four single-rooted periodontally involved human teeth (test groups), and six healthy premolar teeth extracted for orthodontic reasons (control group) were included in this study. A total of 45 root slices were obtained from all selected teeth and assigned to the following five groups: 1) untreated healthy group (+control); 2) untreated periodontally diseased group ( control); 3) hand instrumentation group (scaled Gracey); 4) laser I, Er,Cr:YSGG laser irradiation setting-I (short pulse); and 5) laser II, Er,Cr:YSGG laser irradiation setting-II (long pulse). All of the root slices were autoclaved in phosphate buffered saline and slices were placed onto cell culture inserts. PDL fibroblasts were placed at the density of 80,000 cells on the root plate (5 x 6 mm) and incubated for 48 hours and transferred to 24-well plates. The attachment PDL fibroblasts on the root plates were observed using confocal microscopy (at 12 hours and on days 3 and 7) and scanning electron microscopy (at 12 hours and day 3). 3- (4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay was performed on day 5 for PDL fibroblast survival. Results: 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay shows that whereas laser-treated specimens showed a significantly higher cell density, the Gracey-treated group showed a lower cell density compared to the positive control group (P < 0.05). Based on confocal microscopy, apparent reduction was observed in the attachment of PDL cells to the periodontally diseased root surfaces. In the laser and Gracey groups, cells looked well-oriented to the root surfaces. Laser-treated groups provided suitable environment for cell adhesion and growth. Laser I treatment was more favorable for the attachment of PDL compared to scaled Gracey, laser II, and even healthy root surfaces. Conclusion: The results of the study indicate that short-pulse laser setup (laser I) looks more promising regarding the attachment, spreading, and orientation of PDL cells.en_US
dc.description.sponsorshipSelcuk University, Scientific Research Coordination OfficeSelcuk University [09701419]en_US
dc.description.sponsorshipThis study was performed in the Dental Faculty Research Center of Selcuk University and presented and awarded at the 5th Congress of the International Society for Oral Laser Applications (SOLA), October 22 to 25, 2009, LASER CONGRESS, Vienna, Austria. This project was supported by Selcuk University, Scientific Research Coordination Office (09701419). The authors report no conflicts of interest related to this study.en_US
dc.identifier.citationHakkı, S. S., Korkusuz, P., Berk, G., Dündar, N., Sağlam, M., Bozkurt, B., Purali, N., (2010). Comparison of Er,Cr:YSGG Laser and Hand Instrumentation on the Attachment of Periodontal Ligament Fibroblasts to Periodontally Diseased Root Surfaces: An In Vitro Study. Journal of Periodontology, 81(8), 1216-1225. Doi: 10.1902/jop.2010.090715
dc.identifier.doi10.1902/jop.2010.090715en_US
dc.identifier.endpage1225en_US
dc.identifier.issn0022-3492en_US
dc.identifier.issn1943-3670en_US
dc.identifier.issue8en_US
dc.identifier.pmid20476883en_US
dc.identifier.startpage1216en_US
dc.identifier.urihttps://dx.doi.org/10.1902/jop.2010.090715
dc.identifier.urihttps://hdl.handle.net/20.500.12395/24724
dc.identifier.volume81en_US
dc.identifier.wosWOS:000283023000022en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorHakkı, Sema S.
dc.institutionauthorDündar, Niyazi
dc.institutionauthorBozkurt, Buket
dc.language.isoenen_US
dc.publisherAmer Acad Periodontologyen_US
dc.relation.ispartofJournal of Periodontologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCell biologyen_US
dc.subjectfibroblastsen_US
dc.subjectlasersen_US
dc.titleComparison of Er,Cr:YSGG Laser and Hand Instrumentation on the Attachment of Periodontal Ligament Fibroblasts to Periodontally Diseased Root Surfaces: An In Vitro Studyen_US
dc.typeArticleen_US

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