In vitro micro-propagation of endangered ornamental plant-Neotchihatchewia isatidea (Boiss.) Rauschert
Küçük Resim Yok
Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ACADEMIC JOURNALS
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The ornamental plant, Neotchihatchewia isatidea, is an endangered species of Turkey and threatened by complete extinction in the future. Therefore, in vitro multiplication of this species can be valuable for commercial production and germplasm conservation. Immature embryos of N. isatidea were cultured for initiation on Murashige and Skoog medium ( MS) supplemented with N-6- benzylamino- purine ( BAP) and -naphthaleneacetic acid ( NAA). Shoot primordia were visible within 5 - 6 weeks and the shoot primordia later developed into normal shoots 10 - 12 weeks after the culture initiation on calli developed from immature embryos. Shoot tips were also excised from developed plantlets for direct shoot organogenesis and cultured on MS shoot induction medium supplemented with BAP ( 0.5, 1.0 and 2.0 mg/ l), kinetin ( KIN) ( 0.5, 1.0 and 2.0 mg/ l) and thidiazuron ( TDZ) ( 0.05, 0.10 and 0.50 mg/ l). Direct multiple shoots from shoot tips developed in most media tested. High shoot multiplication ( 3.73), high rooting ( 53 %) number of root per shoot ( 3.66) and survival ratio ( 46.6 %) were achieved.
Açıklama
Anahtar Kelimeler
Neotchihatchewia isatidea, shoot regeneration, immature embryo, shoot tip, rooting.
Kaynak
AFRICAN JOURNAL OF BIOTECHNOLOGY
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
7
Sayı
3
