Photoelectrochemical Biosensing Approach for Alcohol Determination by "Wiring" of Alcohol Oxidase Photonically with P(SNS-NH2)/AOx/CNT/[Ru(bpy)(3)](2+) Modified Electrodes
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
TAYLOR & FRANCIS INC
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A photoelectrochemical alcohol biosensor was designed through photonic wiring of alcohol oxidase (AOx) onto conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine (SNS-NH2) and carbon nanotubes (CNTs) modified gold slides. The photoelectrochemical biosensors utilize a photonically wired electrode that oxidizes primary alcohols to aldehydes. Three different alcohols namely methanol, ethanol, and n-propanol were used as the substrates. In the presence of different concentrations of primary alcohol, the photocurrents were obtained by irradiating of the photoelectrochemical cell containing P(SNS-NH2)/AOx/CNT/[Ru(bpy)(3)](2+) electrode as the anode under air. The bipyridine complex [Ru(bpy)(3)](2+) was used to activate photoinduced electron-transfer reaction and it acted as a redox mediator to activate the bioelectrocatalytic functions of AOx. Therefore, it was shown the photonic electron-transfer wiring of AOx with the electrode. Sensitivity experiments, optimum pH value, operational stabilization and shelf life of the photoelectrochemical biosensor were investigated. Finally, the photoelectrochemical biosensors were used for alcohol detection in real samples.
Açıklama
Anahtar Kelimeler
Photoelectrochemistry, biosensors, alcohol oxidase, primary alcohols., conducting polymers, carbon nanotubes
Kaynak
JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
51
Sayı
5
