Combined voltammetric and spectroscopic investigation of binding interaction between nifedipine and human serum albumin on polyelectrolyte modified ITO electrode

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dc.contributor.authorOzmen, Mustafa
dc.contributor.authorMaltas, Esra
dc.contributor.authorPatir, Imren Hatay
dc.contributor.authorBayrakci, Mevlut
dc.date.accessioned2020-03-26T18:41:18Z
dc.date.available2020-03-26T18:41:18Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe binding interaction between the drug molecule, nifedipine (Nf), and the human serum albumin (HSA) on polyelectrolyte modified indium tin oxide (ITO) electrode has been investigated by the combination of electrochemical and fluorescence spectroscopy techniques. Surface modification has also been characterized by scanning electron microscopy (SEM) and Contact Angle (CA) measurements in each step. The cyclic voltammetry, electrochemical impedance parameters (peak potential difference (Delta E-p)), peak current difference (Delta I-p) and charge-transfer resistance (R-ct) indicate that nifedipine strongly interacted with human serum albumin molecule on the polyelectrolyte modified ITO electrode surface. Stern-Volmer quenching constant K-a is inversely correlated with the temperature, which indicates that the probable quenching mechanism of the nifedipine-human serum albumin binding reaction is initiated by complex formation. The results obtained from these techniques showed that Nf could bind to HSA. The binding constant (K-b) and the number of binding sites (n) of the drug with HSA at different temperatures were determined. At 298 K, K-b was found as 4.04 x 10(-3) and n was 1.08 for Nf-HSA. According to the van't Hoff equation, the thermodynamic parameters, Delta G, Delta H and Delta S, were obtained, showing the involvement of hydrophobic and electrostatic force in this interaction. (C) 2013 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipResearch Foundation of Selcuk University (BAP)Selcuk Universityen_US
dc.description.sponsorshipWe would like to thank The Research Foundation of Selcuk University (BAP) for financial support of this work.en_US
dc.identifier.doi10.1016/j.electacta.2013.08.130en_US
dc.identifier.endpage542en_US
dc.identifier.issn0013-4686en_US
dc.identifier.issn1873-3859en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage535en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.electacta.2013.08.130
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29306
dc.identifier.volume111en_US
dc.identifier.wosWOS:000329531100069en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPERGAMON-ELSEVIER SCIENCE LTDen_US
dc.relation.ispartofELECTROCHIMICA ACTAen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectITO surfaceen_US
dc.subjectPolyelectrolyteen_US
dc.subjectAlbuminen_US
dc.subjectNifedipineen_US
dc.subjectInteractionen_US
dc.titleCombined voltammetric and spectroscopic investigation of binding interaction between nifedipine and human serum albumin on polyelectrolyte modified ITO electrodeen_US
dc.typeArticleen_US

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