Investigation of Insulin-Like Growth Factor Binding Protein 3 (IGFBP-3) Polymorphism in Anatolian Black and Holstein Friesian Cattle Breeds
Yükleniyor...
Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene which is associated with development and growth in livestock. The present study aimed to investigate the polymorphisms of IGFBP-3 gene in Holstein Friesian (HF) and Anatolian Black (AB) cattle breeds. BsuRI (GG?CC) restriction enzyme was used to detect of IGFBP-3 gene polymorphism. Although Anatolian Black breed was monomorphic (AA genotype), three genotypes (AA, AB and BB) were detected in Holstein Friesian breed by digestion of PCR products with BsuRI. The A and B allele frequencies were 0.57 and 0.43, respectively, in Holstein Friesian breed, while A allele frequency was 1.00 in Anatolian Black breed. AA, AB and BB genotype frequencies were 0.32, 0.50 and 0.18, respectively in the Holstein Friesian breed. All three possible genotypes weare detected in Holstein Friesian breed. In the analysis made taking into account Hardy-Weingberg equilibrium, significant deviation was not observed in terms of genotype distributions (P>0.05). In other words, the Holstein Friesian cattle population was found in the Hardy-Weinberg equilibrium.
Açıklama
Anahtar Kelimeler
Anatolian Black, Holstein Friesian, IGFBP-3, PCR-RFLP, BsuRI, Polymorphism
Kaynak
Selcuk Journal of Agriculture and Food Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
34
Sayı
2
Künye
Fadhıl, M., Aytekin, İ., Zülkadir, U., (2020). Investigation of Insulin-Like Growth Factor Binding Protein 3 (IGFBP-3) Polymorphism in Anatolian Black and Holstein Friesian Cattle Breeds. Selcuk Journal of Agriculture and Food Sciences, 34(2), 137-140.
DOI: 10.15316/SJAFS.2020.207
