VECTOR CONSTRUCTION STRATEGIES FOR TRANSFORMATION OF WHEAT PLANT

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dc.contributor.authorBaloglu, M. C.
dc.contributor.authorBattal, A.
dc.contributor.authorAydin, G.
dc.contributor.authorEroglu, A.
dc.contributor.authorOz, M. T.
dc.contributor.authorKavas, M.
dc.contributor.authorOktem, H. A.
dc.date.accessioned2020-03-26T18:44:25Z
dc.date.available2020-03-26T18:44:25Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractGene cloning and vector construction for plant genetic transformation are routine procedures in plant functional genomic studies. The availability of effective transformation vector is one of the pre-requisites for plant transformation studies. Here, we describe the construction of a series of transformation vectors through different genetic engineering techniques for wheat transformation. For this purpose, NAC-type transcription factor genes TaNAC69-1 and TtNAM-B2 were isolated from T. aestivum and T. turgidum, respectively. Then they were cloned into different types of cloning and expression vectors. Besides traditional restriction enzyme digestion and ligation method, Gateway cloning technology which is a fast and reliable alternative cloning method were used for construction of wheat transformation vectors. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium based transformation protocols for wheat plant.en_US
dc.description.sponsorshipResearch Fund of Middle East Technical UniversityMiddle East Technical University [BAP-08-11-DPT2002K120510]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108O786]en_US
dc.description.sponsorshipThis study was supported by the Research Fund of Middle East Technical University Grant No: BAP-08-11-DPT2002K120510 and The Scientific and Technological Research Council of Turkey (TUBITAK) Grant No: 108O786.en_US
dc.identifier.endpage912en_US
dc.identifier.issn1018-7081en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage906en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29992
dc.identifier.volume23en_US
dc.identifier.wosWOS:000321736000037en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPAKISTAN AGRICULTURAL SCIENTISTS FORUMen_US
dc.relation.ispartofJOURNAL OF ANIMAL AND PLANT SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectTraditional gene cloningen_US
dc.subjectGatewaygene cloningen_US
dc.subjectVector constructionen_US
dc.subjectWheat transformationen_US
dc.titleVECTOR CONSTRUCTION STRATEGIES FOR TRANSFORMATION OF WHEAT PLANTen_US
dc.typeArticleen_US

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