Design of an electrochemical biosensing system for xanthine detection and a study on binding interaction of ketoconazole with xanthine oxidase

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dc.contributor.authorBas, Salih Zeki
dc.contributor.authorMaltas, Esra
dc.contributor.authorSennik, Busra
dc.contributor.authorYilmaz, Faruk
dc.date.accessioned2020-03-26T18:49:58Z
dc.date.available2020-03-26T18:49:58Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractXanthine oxidase (XO) was successfully immobilized by covalent attachment on poly(glycidyl methacrylate-co-vinylferrocene) (P(GMA-co-VFc)), a redox copolymer containing pendant epoxy and ferrocene moieties, for the evaluation of both the biosensing properties and the effect of the interaction of ketoconazole (Ktc) with the immobilized XO. The binding interaction between Ktc, a drug used to treat fungal infections, and the immobilized XO on P(GMA-co-VFc) was also studied by fluorescence spectroscopy technique. The binding capacity of the drug was determined using a calibration curve equation that was drawn at excitation wavelength of 300 nm using fluorescence spectroscopy. The interaction ability was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and native-PAGE analysis. The enzyme electrode exhibited a linear range from 2.7 x 10(-3) to 0.55 mM with a sensitivity of 19.42 mu A mM(-1) cm(-2) and a detection limit of 8 x 10(-4) mM for the detection of xanthine. The activation energy (E-a) and the apparent Michaelis-Menten constant (K-mapp) values were found to be 12.30 kJ mol(-1) and 0.38 mM, respectively. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific Research Projects of Selcuk UniversitySelcuk Universityen_US
dc.description.sponsorshipThe authors are grateful for the financial support by the Scientific Research Projects of Selcuk University.en_US
dc.identifier.doi10.1016/j.colsurfa.2013.12.030en_US
dc.identifier.endpage47en_US
dc.identifier.issn0927-7757en_US
dc.identifier.issn1873-4359en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage40en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.colsurfa.2013.12.030
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30722
dc.identifier.volume444en_US
dc.identifier.wosWOS:000333000800006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofCOLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectXanthine oxidaseen_US
dc.subjectProtein-drug interactionen_US
dc.subjectKetoconazoleen_US
dc.subjectBiosensoren_US
dc.subjectFluorescenceen_US
dc.titleDesign of an electrochemical biosensing system for xanthine detection and a study on binding interaction of ketoconazole with xanthine oxidaseen_US
dc.typeArticleen_US

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