Boğalarda Bazı Önemli? Patojen Etkenleri?n Araştırılması
Yükleniyor...
Dosyalar
Tarih
2004
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, boğa semen ve propusyal y kanti omeklerinde, Bovine Herpesvirus 1 (BHV-1), Bovine Viral Diarrhae Virus (BVDV). Campylobacter. Brucella ve Mycoplasma gibi bazı önemli enfeksiyoz etkenlerin varlığı, viral/bakteriyel izolasyon, PCR/RT-PCR ve serolojik yöntemlerle araştırıldı Çalışmada, mezbahaya kesime gelinlen bir yaşın üzerindeki hayvanlardan alınan 73 adet semen. 94 adet prepusyal yıkantı ve 101 adet kan serumu örneği ile bir suni tohumlama merkezindeki 19 adet boğadan değişik tanhirde alınan 210 adet dondurulmuş semen ve bu hayvanların kan serumu örnekleri incelendi. Semen örneklerinden PCR/RT-PCR ve virus izolasyon yöntemlen ile BHV-1 ve BVDV, bakteriyolojik muayenelerde ise Brucella ve Campylobacter etkenlen tespit edilmedi. Incelenen 94 prepusyal yıkantı örneğinin 24 (%25.5) unden Mycoplasma spp. izole edildu Serolojik olarak ELISA ile test edilen 120 boğaya ait (19'u suni tohumlama merkezi, 1011 mezbahadan alinan) kan serumlanının 42'sinde (35) BHV-1, 46 (39.6)'sinda BVDV antikorfan tespit edildi. Suni tohumlama merkezine ait 19 boğa kan serumunun tumu BHV-1. 4'ü ise BVDV antikor pozitif bulundu. Incelenen serumlanın tümünde Brucella antikoran negatif bulundu. Çalişmada test edilen hayvanların hiçbinnde klinik olarak herhangi bir bulguya rastlanmadi.
In this study, presence of some important pathogens such as Bovine Herpesvirus 1 (BHV-1), Bovine Viral De arrhea Vinus (BVDV), Campylobacter. Brucella and Mycoplasma were investigated by viral and bactenological isolation, PCR/RT-PCR and serological methods in bulls. Seventy three semen 94 prepubial washings and 101 serum samples from bulls above 1 year old brought in slaughterhouse and 210 extended semen samples collected in different dates from 19 bulls in an artificial insemination center and their sera were examined. BHV-1 and BVDV were not determined by PCR/RTPCR and virus isolation in semen samples. Brucella and Campylobacter were not isolated in any of semen and preputial washing samples. Mycoplasma spp was isolated in 24 (25.5%) of 94 preputial washing samples in 120 bull sera (19 sera from an artificial insemination center, 101 from slaughterhouse) tested by ELISA, 42 (35 %) were positive for BHV-1 and 46 (396%) for BVDV antibodies of the 19 bulls in an artificial insemination center all were scropositive for BHV-1 and 4 were seropositive for BVDV All serum samples were negative for Buceta antibodies. None of the bulls examined in this study had any clinical symptoms
In this study, presence of some important pathogens such as Bovine Herpesvirus 1 (BHV-1), Bovine Viral De arrhea Vinus (BVDV), Campylobacter. Brucella and Mycoplasma were investigated by viral and bactenological isolation, PCR/RT-PCR and serological methods in bulls. Seventy three semen 94 prepubial washings and 101 serum samples from bulls above 1 year old brought in slaughterhouse and 210 extended semen samples collected in different dates from 19 bulls in an artificial insemination center and their sera were examined. BHV-1 and BVDV were not determined by PCR/RTPCR and virus isolation in semen samples. Brucella and Campylobacter were not isolated in any of semen and preputial washing samples. Mycoplasma spp was isolated in 24 (25.5%) of 94 preputial washing samples in 120 bull sera (19 sera from an artificial insemination center, 101 from slaughterhouse) tested by ELISA, 42 (35 %) were positive for BHV-1 and 46 (396%) for BVDV antibodies of the 19 bulls in an artificial insemination center all were scropositive for BHV-1 and 4 were seropositive for BVDV All serum samples were negative for Buceta antibodies. None of the bulls examined in this study had any clinical symptoms
Açıklama
Anahtar Kelimeler
Boğa, Semen, BHV-1, BVDV, PCR, RT-PCR, Campylobacter, Bull, Mycoplasma, Brucella
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
20
Sayı
4
Künye
Güler, L., Ok, Ü., Şimşek, A., Yavru, S., Ekik, M., Gündüz, K., Gülcü, Y., (2004). Boğalarda Bazı Önemli̇ Patojen Etkenleri̇n Araştırılması. Eurasian Journal of Veterinary Sciences, 20(4), 71-78.
