Effect of Different Storage Temperatures on the Stability of Bovine Viral Diarrhea Virus RNA in Blood Samples

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dc.contributor.authorAvci, Oguzhan
dc.contributor.authorBulut, Oya
dc.contributor.authorYapici, Orhan
dc.contributor.authorSimsek, Atilla
dc.contributor.authorYavru, Sibel
dc.contributor.authorDik, Irmak
dc.contributor.authorAtli, Kamil
dc.date.accessioned2020-03-26T19:02:03Z
dc.date.available2020-03-26T19:02:03Z
dc.date.issued2015
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe present study was conducted to determine stability of Bovine viral diarrhea virus (BVDV) RNA stored at different temperatures. A total of 6 blood samples obtained from a private cattle farm, which were found to be antigen positive (Ag+) by direct ELISA method, were used in this study. BVDV Ag+ samples were stored separately at 4, 21 and 37 degrees C for 1 month. The samples were analyzed on the 0, 1(st), 2(nd), 3(rd) and 4(th) weeks by ELISA for the presence of BVDV Ag and by RT-PCR for the presence of BVDV RNA. Stability of BVDV RNA was calculated using maximum concentration (C-max) and area under the curve (AUC) as kinetic parameters of each sample. All of the samples were found positive both by ELISA and RT-PCR on each week. Cmax values of BVDV RNA for the storage temperatures of 4, 21 and 37 degrees C were 356, 346 and 338 ng/mu L respectively, and AUC(0 -> 4) values for the same temperatures were 1151, 1106 and 1077 week. ng/mu L respectively. It was determined that storage at different temperatures for one month does not statistically influence the kinetic parameters of BVDV RNA (P>0.05). In conclusion, it can be expressed that storage of BVDV RNA at 4, 21 and 37 degrees C for one month has no effect on the stability of BVDV RNA. (C) 2015 PVJ. All rights reserveden_US
dc.identifier.endpage530en_US
dc.identifier.issn0253-8318en_US
dc.identifier.issn2074-7764en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage528en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/32035
dc.identifier.volume35en_US
dc.identifier.wosWOS:000366758200028en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUNIV AGRICULTURE, FAC VETERINARY SCIENCEen_US
dc.relation.ispartofPAKISTAN VETERINARY JOURNALen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAUCen_US
dc.subjectBVDVen_US
dc.subjectC-maxen_US
dc.subjectELISAen_US
dc.subjectRT-PCRen_US
dc.titleEffect of Different Storage Temperatures on the Stability of Bovine Viral Diarrhea Virus RNA in Blood Samplesen_US
dc.typeArticleen_US

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