MDA Level in Colon Tissue of the Rats with Colon Cancer Model Treated with Diallyl Disulfide
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Dosyalar
Tarih
2023 Haziran
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Sunulan çalışma azoksimetan ile kolon kanseri oluşturulan ratlarda sarımsağın biyoaktif bileşiklerinden biri olan dialil disülfid’in, kolon dokusu malondialdehit seviyesi üzerine etkisini araştırmak amacı ile yapıldı. Gereç ve Yöntem: Çalışmada 58 adet erkek Wistar Albino rat kullanıldı. Hayvanlar 5 gruba ayrıldı. 1. grup kontrol grubu (n=12), 2. grup azoksimetan (15mg/kg vücut ağırlığı) uygulanan kanser grubu (n=12), 3. grup sadece dialil disülfit (DADS, 50 mg/kg vücut ağırlığı) uygulanan (n=11), 4. grup azoksimetan ve dialil disülfit uygulanan (n=11) ve 5. grup ise taşıt madde olan mısır yağının (1ml/kg vücut ağırlığı) verildiği gruptur (n=12). Deneme süresi olan 18 haftanın sonunda servikal dislokasyon ile hayvanlar sakrifiye edildi ve kolon dokuları çıkarıldı. Alınan dokuların homojenizasyonu sağlandı ve ELISA cihazı ile kite uygun prosedür kullanılarak MDA seviyeleri ölçüldü. Bulgular: Dokularda tümör oluşumu makroskobik olarak tespit edildi. Yapılan analiz sonucu gruplar arasında MDA seviyesi yönünden herhangi bir fark bulunmadığı belirlendi (P>0.05). Öneri: Sonuç olarak çalışma koşullarında rat kolon dokusunda DADS veya azoksimetan uygulanasının lipid peroksidasyonuna neden olmadığı ifade edilebilir. DADS veya AOM’nin kolon dokusundaki etkinliği, hayvanlara uygulanma şekli ve miktarı göz önüne alınarak ileride yapılacak olan çalışmalara ihtiyaç vardır.
Aim: This study was conducted to investigate the effect of diallyl disulfide, one of the bioactive compounds of garlic, on the malondialdehyde level of colon tissue in rats with azoxymethane-induced colon cancer. Materials and Methods: A total of 58 male Wistar Albino rats were used in the study. The animals were divided into 5 groups. The first group was the control group (n=12), the second group was the cancer group (n=12) treated with azoxymethane (15 mg/kg body weight), the third group was the group to whom only diallyl disulfide (DADS, 50 mg/kg body weight) was administered (n=11), the fourth group was the group to whom azoxymethane and diallyl disulfide were administered (n=11) and the fifth group was the group to whom corn oil (1 ml/kg body weight) was administered as carrier substance (n=12). The experimental the one which period at the end of 18 weeks, the animals were sacrificed by cervical dislocation and their colon tissues were excised. The tissues were homogenized and MDA levels were measured with the ELISA device using the procedure, suitable for the kit. Results: Tumor formation in the tissues was macroscopically identified. The analysis results indicated no difference between the groups with respect to their MDA levels (P>0.05). Conclusion: In conclusion, it can be asserted that the administration of DADS or azoxymethane did not cause lipid peroxidation in the rat colon tissue under the experimental conditions. There is a need for further studies investigating the effectiveness of DADS or AOM in colon tissue by taking the method and the dose of treatment to the animals into consideration.
Aim: This study was conducted to investigate the effect of diallyl disulfide, one of the bioactive compounds of garlic, on the malondialdehyde level of colon tissue in rats with azoxymethane-induced colon cancer. Materials and Methods: A total of 58 male Wistar Albino rats were used in the study. The animals were divided into 5 groups. The first group was the control group (n=12), the second group was the cancer group (n=12) treated with azoxymethane (15 mg/kg body weight), the third group was the group to whom only diallyl disulfide (DADS, 50 mg/kg body weight) was administered (n=11), the fourth group was the group to whom azoxymethane and diallyl disulfide were administered (n=11) and the fifth group was the group to whom corn oil (1 ml/kg body weight) was administered as carrier substance (n=12). The experimental the one which period at the end of 18 weeks, the animals were sacrificed by cervical dislocation and their colon tissues were excised. The tissues were homogenized and MDA levels were measured with the ELISA device using the procedure, suitable for the kit. Results: Tumor formation in the tissues was macroscopically identified. The analysis results indicated no difference between the groups with respect to their MDA levels (P>0.05). Conclusion: In conclusion, it can be asserted that the administration of DADS or azoxymethane did not cause lipid peroxidation in the rat colon tissue under the experimental conditions. There is a need for further studies investigating the effectiveness of DADS or AOM in colon tissue by taking the method and the dose of treatment to the animals into consideration.
Açıklama
Anahtar Kelimeler
AOM, dialil disülfid, kolon, MDA, rat, colon, diallyl disulfide
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
39
Sayı
2
Künye
Alan, B. S., Bulut, Z., (2023). MDA Level in Colon Tissue of the Rats with Colon Cancer Model Treated with Diallyl Disulfide. Eurasian Journal of Veterinary Sciences, 39(2), 100-105.
DOI: 10.15312/EurasianJVetSci.2023.404
