Comparison of steroid and platelet-rich plasma (PRP) applications in the treatment of collagenase induced tendinopathy in rabbit
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Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
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Yayıncı
Selçuk Üniversitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada, tavşanlarda kollajenaz (Kollajenaz Tip I) ile oluşturulmuş aşil tendinopatilerinde trombositten zengin plazma (PRP) ve MPA (metilprednizolon asetat) enjeksiyonlarının terapötik etkilerini histopatolojik olarak değerlendirmeyi amaçlamıştır. Gereç ve Yöntem: Aşil tendinopatisi, 23 tavşanın sağ ve sol aşil tendonlarının proksimal ve distal bölgelerine intratendinöz tip I kollajenaz enjeksiyonu ile oluşturuldu. Kolajenaz enjeksiyonundan bir hafta sonra, sağ aşil tendonları- nın distaline PRP ve sol aşil tendonlarının distaline MPA enjekte edildi. Tüm aşil tendonlarının proksimal kısımlarına %0,9 serum fizyolojik enjekte edildi. Tavşanlar, terapötik enjeksiyonlardan 3, 6 ve 8 hafta sonunda ötenazi edildi. Tendonların histolojik dejenerasyon derecesi Modifiye Movin skorlamasına göre yapıldı. Bulgular: Tedavi grupları ile % 0,9’luk serum fizyolojik enjekte edilen grup arasında incelenen tüm dönemlerde istatistiksel açıdan önemli farklılıklar tespit (p<0,05) edildi. Altıncı hafta sonunda tedavi grupları arasında tüm parametrelerde istatistiksel olarak önemli olmayan orta düzeyde iyileşme gözlenirken, 8. hafta sonunda MPA uygulaması yapılan tavşanlarla kıyasla PRP enjeksiyonu uygulanan hayvanların aşil tendonlarında kollajen ipliklerin paralel düzenlenmeleri ve demetleşmenin daha iyi olduğu gözlendi. Ayrıca tedavi grupları 8. hafta sonunda karşılaştırıldığında, PRP grubunun vaskülarizasyon ve tenosit çekirdek parametrelerinde istatistiksel olarak önemli düzeyde iyileşme gözlendi (p<0,05). Öneri: PRP enjeksiyonlarının, MPA enjeksiyonuna göre tendinopati tedavisinde daha erken iyileşme sağlayacağı kanısındayız.
Aim: The aim of this study was to evaluate histopathologically the therapeutic effects of platelet-rich plasma (PRP) and MPA (methylprednisolone acetate) injections in rabbits achill tendinopathies induced by collagenase (Collagenase Type I) Materials and Methods: Achilles tendinopathy was formed with intrathendinous injections of type I collagenase into the proximal and distal regions of the right and left achilles tendons of 23 rabbits. PRP was injected distal to the right Achilles tendons and MPA to the distal of the left Achilles tendons. A 0,9% saline solution was also injected into the proximal parts of all achilles tendons. Rabbits were euthanized 3, 6, and 8 weeks after the therapeutic injections. The degree of histopathological degeneration of the tendons was made according to the Modified Movin scoring. Results: Statistically significant differences were detected between the treatment groups and the 0.9% saline injected group (p<0.05). In the histopathological evaluations performed at the end of the 6th week no statistically significant difference was detected between the treatment groups (p>0.05) without tenocyte nuclei (p<0.05). At the end of the 8 weeks vascularization and tenocyte nuclei (p<0.05) parameters was observed better in the Achilles tendons of the rabbits undergoing PRP injection compared to rabbits with MPA application. Conclusion: In conclusion, we consider that PRP injections tend to provide an earlier improvement in the treatment of tendinopathy compared to MPA injection.
Aim: The aim of this study was to evaluate histopathologically the therapeutic effects of platelet-rich plasma (PRP) and MPA (methylprednisolone acetate) injections in rabbits achill tendinopathies induced by collagenase (Collagenase Type I) Materials and Methods: Achilles tendinopathy was formed with intrathendinous injections of type I collagenase into the proximal and distal regions of the right and left achilles tendons of 23 rabbits. PRP was injected distal to the right Achilles tendons and MPA to the distal of the left Achilles tendons. A 0,9% saline solution was also injected into the proximal parts of all achilles tendons. Rabbits were euthanized 3, 6, and 8 weeks after the therapeutic injections. The degree of histopathological degeneration of the tendons was made according to the Modified Movin scoring. Results: Statistically significant differences were detected between the treatment groups and the 0.9% saline injected group (p<0.05). In the histopathological evaluations performed at the end of the 6th week no statistically significant difference was detected between the treatment groups (p>0.05) without tenocyte nuclei (p<0.05). At the end of the 8 weeks vascularization and tenocyte nuclei (p<0.05) parameters was observed better in the Achilles tendons of the rabbits undergoing PRP injection compared to rabbits with MPA application. Conclusion: In conclusion, we consider that PRP injections tend to provide an earlier improvement in the treatment of tendinopathy compared to MPA injection.
Açıklama
Anahtar Kelimeler
Aşil tendinopatisi, kolajenaz Tip I, metilprednizolon, PRP, steroid, Achilles tendinopathy, collagenase Type I, methylprednisolone, steroid
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
37
Sayı
2
Künye
Zamirbekova, N., Yavru, N., (2021). Comparison of steroid and platelet-rich plasma (PRP) applications in the treatment of
collagenase induced tendinopathy in rabbit. Eurasian Journal of Veterinary Sciences, 37 (2), 82-92. DOI: 10.15312/EurasianJVetSci.2021.330
