Ergothioneine attenuates the DNA damage of post-thawed Merino ram sperm

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dc.contributor.authorCoyan, Kenan
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorBaspinar, Nuri
dc.contributor.authorTaspinar, Mehmet
dc.contributor.authorAydos, Sena
dc.date.accessioned2020-03-26T18:25:31Z
dc.date.available2020-03-26T18:25:31Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe objective of the current study was to evaluate the effects of antioxidant ergothioneine added to cryopreservation extender on the DNA integrity of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2 years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing ergothioneine at different concentrations and no antioxidant (control), were cooled to 5 degrees C and frozen in 0.25 ml French straws. Frozen straws were then thawed at 37 degrees C for 20s in a water bath for evaluation of sperm DNA damage using the Comet test. The addition of ergothioneine at concentrations of 1, 2 and 4 mM resulted in lower sperm with damaged DNA (5.4, 4.7 and 3.2%, respectively) than that of control (7.9%), thus reducing the DNA damage (P<0.01). Findings of this study showed that the increasing concentrations of ergothioneine in semen extenders, were of greater benefit to DNA integrity of frozen-thawed ram sperm. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipSelcuk University, Directorate of Scientific Research Projects (BAP)Selcuk University; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108 O 522]en_US
dc.description.sponsorshipThe study was financed under the project supported by Selcuk University, Directorate of Scientific Research Projects (BAP) and by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 108 O 522).en_US
dc.identifier.doi10.1016/j.smallrumres.2012.02.002en_US
dc.identifier.endpage167en_US
dc.identifier.issn0921-4488en_US
dc.identifier.issue02.03.2020en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage165en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.smallrumres.2012.02.002
dc.identifier.urihttps://hdl.handle.net/20.500.12395/28018
dc.identifier.volume106en_US
dc.identifier.wosWOS:000307688800016en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofSMALL RUMINANT RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectDNA integrityen_US
dc.subjectErgothioneineen_US
dc.subjectFreezing-thawingen_US
dc.subjectRam spermen_US
dc.titleErgothioneine attenuates the DNA damage of post-thawed Merino ram spermen_US
dc.typeArticleen_US

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