Atomic Force Microscopy Study of Living Baker's Yeast Cells

dc.contributor.authorMikoliunaite, L.
dc.contributor.authorMakaraviciute, A.
dc.contributor.authorSuchodolskis, A.
dc.contributor.authorRamanaviciene, A.
dc.contributor.authorOztekin, Y.
dc.contributor.authorStirke, A.
dc.contributor.authorJurkaite, G.
dc.date.accessioned2020-03-26T18:13:50Z
dc.date.available2020-03-26T18:13:50Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractCurrently, Atomic Force Microscopy (AFM) has a wide range of applications in a variety of disciplines of science and industry including biology and medicine. Despite the maturity of the AFM technique, it is still finding new opportunities in visualization of biological materials and biomolecular processes. The most frequent and ordinary application of AFM is surface topology and morphological studies. This paper describes educational application of AFM in observation of a living cell wall structure. For the following study we have selected Sacchoromyces cerevisiae cells, better known as baker's yeast cells that are available in any supermarket and widely used for fermentation control of food and drinks, and as a leavening agent in baking. The sample preparation and measurements are described. Living yeast cells were prepared in solutions containing glucose or sodium chloride. Proposed AFM based protocol allows the researcher to keep baker's yeast cells alive, and to observe them in air conditions. The experiment is suitable for Master and/or Ph.D. students and is designed to show the main principles of the Bio-AFM operation. In some particular cases, this protocol may be adoptable for bachelor students, who have already completed an extended course in nanotechnology, biotechnology or microbiology. To show educational suitability, the protocol has been tested in the environment of educational laboratory and performed by students studying physics, chemistry, biotechnology and biology.en_US
dc.description.sponsorshipLithuanian State Science and Studies Foundation [S-19/2008]; Research Council of Lithuania [MOS-9/2010]en_US
dc.description.sponsorshipThe study was supported by Lithuanian State Science and Studies Foundation according to contract number S-19/2008 and by Research Council of Lithuania according to contract number MOS-9/2010.en_US
dc.identifier.doi10.1166/asl.2011.1236en_US
dc.identifier.endpage376en_US
dc.identifier.issn1936-6612en_US
dc.identifier.issn1936-7317en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage368en_US
dc.identifier.urihttps://dx.doi.org/10.1166/asl.2011.1236
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26176
dc.identifier.volume4en_US
dc.identifier.wosWOS:000291410900015en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAMER SCIENTIFIC PUBLISHERSen_US
dc.relation.ispartofADVANCED SCIENCE LETTERSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEducationen_US
dc.subjectNanotechnolgyen_US
dc.subjectNanobiotechnologyen_US
dc.subjectAFMen_US
dc.subjectYeasten_US
dc.subjectLiving Cellen_US
dc.titleAtomic Force Microscopy Study of Living Baker's Yeast Cellsen_US
dc.typeArticleen_US

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