Effects of Drugs on Kinetic Values of Cytokines, Adenosine Deaminase and 13,14-dihydro-15-keto-prostaglandin F? in Endotoxemia: A Different Approach 2
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Dosyalar
Tarih
2010
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Cilt Başlığı
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Lipopolisakkaritle (LPS) oluşturulan deneysel endotoksemide sitokinler, adenozin deaminaz (ADA) ve 13,14-dihidro-15-keto-prostaglandin F2? (PGM)’nın kinetik değerlerine tek başlarına ve/veya kombine uygulanan enrofloksasin (ENR), fluniksin meglumin (FM) ve deksametazonun (DEX) etkilerini belirlemektir. Gereç ve Yöntem: Araştırmada kullanılan ratlar 7 gruba ayrıldı. Deneysel endotoksemi oluşturmak için pozitif kontrol grubu dahil bütün gruplara LPS uygulandı. Diğer altı gruba ENR, FM, düşük doz DEX, yüksek doz DEX, ENR+FM+düşük doz DEX ve ENR+FM+yüksek doz DEX uygulandı. Uygulama sonrası 0, 1, 2, 4, 6, 8, 12, 24 ve 48. saatlerde kan örnekleri toplandı. Tümör nekroz faktör alfa (TNF?), interlökin-6 (IL6), interlökin-10 (IL-10), ADA ve PGM düzeyleri ELISA ile belirlendi. Eğri altında kalan alan (EAA0-48) farmakokinetik programla, plazma veya serum maksimum konsantrasyon (Cmax) ile maksimum konsantrasyona ulaşma zamanı (tmax) direk bakı yöntemiyle belirlendi. Bulgular: Pozitif kontrol (LPS) grubuyla kıyaslandığında EAA0-48 değerlerinin; ENR grubunda PGM için artarken (p<0.05), IL-6, IL-10 ve ADA için azaldığı (p<0.05); FM grubunda IL-6 ve ADA’ya özgü olarak küçüldüğü (p<0.05); DEX tek başına veya kombine uygulandığı gruplarda da azaldığı (p<0.05) belirlendi. Öneri: Farklı örnekleme zamanlarında çok sayıda ölçülen aynı endotoksemi belirteçlerinin toplu değerlendirilmesinde kinetik parametrelerden özellikle EAA’nin farklı ve akılcı bir yaklaşım olarak dikkate alınabileceği kanaatine varıldı.
Aim: The objective of this study was to determine the effects of enrofloxacin (ENR), flunixin meglumine (FM) and dexamethasone (DEX) on kinetic values of cytokines, adenosine deaminase (ADA) and 13,14-dihydro-15-keto-prostaglandin F2a (PGM) in lipopolysaccharide-induced endotoxemia. Materials and Methods: Rats were divided into seven groups. To induce endotoxemia, lipopolysaccharide (LPS) was injected into all groups, including the positive control. The six other groups received the following drugs: ENR, FM, low-dose DEX, high-dose DEX, ENR + FM + low-dose DEX and ENR + FM + high-dose DEX. After the treatments, blood samples were collected at 0, 1, 2, 4, 6, 8, 12, 24, and 48 hours. Serum tumor necrosis factor alpha (TNF?), interleukin-6 (IL-6), interleukin-10 (IL-10), ADA and plasma PGM levels were determined by ELISA. Area under the concentration time curve (AUC0-48), maximal concentration in plasma or serum (Cmax) and time to reach (tmax) values were determined by pharmacokinetic computer program. Results: ENR increased (p<0.05) AUC0-48 of PGM and decreased (p<0.05) AUC0-48 of IL-6, IL-10 and ADA, while FM decreased (p<0.05) AUC0-48 of IL-6 and ADA compared to LPS group. DEX alone and combined administrations caused the lower AUC0-48 of all values (p<0.05). Conclusion: Kinetic values, especially AUC, may be used for total evaluation of endotoxemia markers determined at different sampling times in same groups as a different and logical approach.
Aim: The objective of this study was to determine the effects of enrofloxacin (ENR), flunixin meglumine (FM) and dexamethasone (DEX) on kinetic values of cytokines, adenosine deaminase (ADA) and 13,14-dihydro-15-keto-prostaglandin F2a (PGM) in lipopolysaccharide-induced endotoxemia. Materials and Methods: Rats were divided into seven groups. To induce endotoxemia, lipopolysaccharide (LPS) was injected into all groups, including the positive control. The six other groups received the following drugs: ENR, FM, low-dose DEX, high-dose DEX, ENR + FM + low-dose DEX and ENR + FM + high-dose DEX. After the treatments, blood samples were collected at 0, 1, 2, 4, 6, 8, 12, 24, and 48 hours. Serum tumor necrosis factor alpha (TNF?), interleukin-6 (IL-6), interleukin-10 (IL-10), ADA and plasma PGM levels were determined by ELISA. Area under the concentration time curve (AUC0-48), maximal concentration in plasma or serum (Cmax) and time to reach (tmax) values were determined by pharmacokinetic computer program. Results: ENR increased (p<0.05) AUC0-48 of PGM and decreased (p<0.05) AUC0-48 of IL-6, IL-10 and ADA, while FM decreased (p<0.05) AUC0-48 of IL-6 and ADA compared to LPS group. DEX alone and combined administrations caused the lower AUC0-48 of all values (p<0.05). Conclusion: Kinetic values, especially AUC, may be used for total evaluation of endotoxemia markers determined at different sampling times in same groups as a different and logical approach.
Açıklama
Anahtar Kelimeler
fluniksin, Enrofloksasin, deksametazon, kinetik, endotoksemi belirteçleri, Enrofloxacin, flunixin, dexamethasone, kinetic, endotoxemia markers
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
26
Sayı
1
Künye
Altan, F., Elmas, M., Er, A., Üney, K., Çetim, G., Traş, B., Yazar, E., (2010). Effects of Drugs on Kinetic Values of Cytokines, Adenosine Deaminase and 13,14-dihydro-15-keto-prostaglandin F_{2\alpha} in Endotoxemia: a Different Approach. Eurasian Journal of Veterinary Sciences, 26(1), 15-19.
