Leptin and IGF-I improve bovine embryo quality in vitro

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dc.contributor.authorKaya, Abdullah
dc.contributor.authorSagirkaya, Hakan
dc.contributor.authorMisirlioglu, Muge
dc.contributor.authorGumen, Ahmet
dc.contributor.authorParrish, John J.
dc.contributor.authorMemili, Erdogan
dc.date.accessioned2020-03-26T19:42:00Z
dc.date.available2020-03-26T19:42:00Z
dc.date.issued2017
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe in vitro embryo culture systems need further improvement to enhance the efficiency of bovine embryo production. Growth factors play key roles in embryo production and quality. The objective of this study was to define the effects of leptin, insulin-like growth factor-1 (IGF-1), and their combination on embryonic development, apoptosis, and expression profiles of a panel of developmentally important genes during 8-day embryo culture. The oocytes were aspirated from slaughterhouse ovaries of mixed breed cows. Following IVM/IVF presumptive zygotes were obtained. To accomplish this objective, presumptive zygotes (16-18 h post-insemination) were cultured in vitro as control (no supplementation, n = 349), 5 ng/ml leptin (Group I, n = 322), 100 ng/ml IGF-1 (Group II, n = 347), and 5 ng/ml leptin and 100 ng/ml IGF-1 (Group III, n = 360). All groups were supplemented with 10% fetal calf serum (FCS) on Day 4, and blastocysts were harvested on day 8. The DNA-fragmented nuclei of blastocyst were determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and expression profiles of a panel of developmentally important genes were assayed by real-time polymerase chain reaction (RT-PCR). The cleavage rate and embryo development to 8-16 cell stage were higher in groups II and III as compared to control (P < 0.05), respectively. Percentage of blastocyst and mean cell numbers per blastocyst did not differ among the groups. Addition of IGF-I and/or combination with leptin decreased the number of nuclei with fragmented DNA (P < 0.01) as compared to the control group. Although the expression of glucose transporter 1 (Glut1), desmosomal glycoprotein desmocollin III (DcIII), and insulin like growth factor 2 receptor (Igf2r) transcripts did not change among the groups, interferon-tau (IF-tau) and DNA methyltransferase 3A (Dnmt3a) were down-regulated in group II while heat shock protein-70 (Hsp70) and IF-tau were up regulated in group III. Results indicate that addition of IGF-I in culture media improved the cleavage rate; combination with leptin also improved the development rates to 8-16-cell-stage embryos, decreased the TUNEL-positive nuclei, and caused alterations in the amounts of transcripts for the developmentally important genes assayed.en_US
dc.description.sponsorshipUniversity of Wisconsin-Madison College of Agriculture and Life Sciences; Mississippi Agriculture Forestry and Experiment Station, USA; IDB; University of Selcuk, Konya, Turkeyen_US
dc.description.sponsorshipAuthors would like to thank Mr. Brad Haley for help in obtaining bovine ovaries and Mr. Peter Crump for statistical analysis of the data. Research was supported by the University of Wisconsin-Madison College of Agriculture and Life Sciences, and by Mississippi Agriculture Forestry and Experiment Station, USA. A. Kaya was supported by IDB and by University of Selcuk, Konya, Turkey.en_US
dc.identifier.doi10.21451/1984-3143-AR987en_US
dc.identifier.endpage1160en_US
dc.identifier.issn1806-9614en_US
dc.identifier.issn1984-3143en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1151en_US
dc.identifier.urihttps://dx.doi.org/10.21451/1984-3143-AR987
dc.identifier.urihttps://hdl.handle.net/20.500.12395/35255
dc.identifier.volume14en_US
dc.identifier.wosWOS:000416207600017en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBRAZILIAN COLL ANIMAL REPRODUCTIONen_US
dc.relation.ispartofANIMAL REPRODUCTIONen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectbovineen_US
dc.subjectpreimplantation developmenten_US
dc.subjectapoptosisen_US
dc.subjectleptinen_US
dc.subjectIGF-Ien_US
dc.titleLeptin and IGF-I improve bovine embryo quality in vitroen_US
dc.typeArticleen_US

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