CHARACTERIZATION OF ESCHERICHIA COLI STRAINS ISOLATED FROM WELL WATERS: MOLECULAR TYPING BY PULSED-FIELD GEL ELECTROPHORESIS, ANTIBIOTIC RESISTANCE PATTERNS AND PLASMID PROFILES

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dc.contributor.authorUysal, Ahmet
dc.contributor.authorDurak, Yusuf
dc.contributor.authorArslan, Ugur
dc.date.accessioned2020-03-26T18:41:15Z
dc.date.available2020-03-26T18:41:15Z
dc.date.issued2013
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractA number of previous studies have shown that characterization and determination of genetic relationships of the microorganisms in case of possible outbreak are of vital importance. In this study, the genetic relations and genetic diversities, susceptibility to antibiotics and plasmid profiles of 43 Escherichia coli isolates recovered from well water samples were investigated.. The pulsed-field gel electrophoresis (PFGE) method was used to identify the genetic relations and diversities of E. coli isolates. PFGE revealed 30 pulsotypes represented by 6 subtypes among the strains according to evaluation of restriction profiles. Antibiotic susceptibility tests were conducted against 15 antibiotics by using a disc diffusion method. The isolates exhibited four different types of resistance profiles. The strains showed the greatest resistance to ampicillin (97.67%), followed by ticarcillin-clavulanic acid (9.3%) and cefuroxime and ceftazidime (6.97%). Plasmid isolation studies of the strains conducted by the method of alkaline lysis revealed that 19 (44.18%) of 40 E. coli strains contain 19 different plasmid bands ranging between 78.2 and 2.6 kb. Based on the results obtained from tests, PFGE analysis revealed very high genetic diversity among the strains. Antibiotic resistance ratios increased in E. coli isolates when compared with data obtained from previous studies. Plasmids of E. coli strains demonstrated random distribution, and any significant correlation between antibiotic resistance patterns and plasmids has not been found. E. coli strains leaked to the well water sources were not closely related. Studies and surveillances should be conducted periodically to see resistance of environmental strains.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordinating Office (BAP)Selcuk University [10101022]en_US
dc.description.sponsorshipWe would like to thank Selcuk University Scientific Research Projects Coordinating Office (BAP) for supporting this project financially (Project No: 10101022). We also thank Izmir Institute of Technology, Biotechnology and Bioengineering Research and Application Centre for their technical help.en_US
dc.identifier.endpage3533en_US
dc.identifier.issn1018-4619en_US
dc.identifier.issn1610-2304en_US
dc.identifier.issue12en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage3525en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/29286
dc.identifier.volume22en_US
dc.identifier.wosWOS:000329084600011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPARLAR SCIENTIFIC PUBLICATIONS (P S P)en_US
dc.relation.ispartofFRESENIUS ENVIRONMENTAL BULLETINen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEscherichia colien_US
dc.subjectantibiotic resistanceen_US
dc.subjectplasmid profileen_US
dc.subjectpulsed field gel electrophoresis (PFGE)en_US
dc.subjectwell wateren_US
dc.titleCHARACTERIZATION OF ESCHERICHIA COLI STRAINS ISOLATED FROM WELL WATERS: MOLECULAR TYPING BY PULSED-FIELD GEL ELECTROPHORESIS, ANTIBIOTIC RESISTANCE PATTERNS AND PLASMID PROFILESen_US
dc.typeArticleen_US

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