Fomes fomentarius and tricholoma anatolicum (Agaricomycetes) extracts exhibit significant multiple drug-resistant modulation activity in drug-resistant breast cancer cells
Küçük Resim Yok
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Begell House Inc.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Multiple drug resistance is one of the main problems that hinder successful cancer chemotherapy. Investigations on the development of effective chemotherapeutic agents and drug resistance inhibitors motivate studies on the effects of natural compounds on drug-resistant cancer cells. For this purpose, aqueous, methanol, and ethanol extracts of Fomes fomentarius and Tricholoma anatolicum were prepared. The extracts were evaluated to assess their anticancer and multiple drug resistance modulation activities. Cytotoxic effects of F. fomentarius and T. anatolicum extracts on paclitaxel and vincristine resistant P-glycoprotein over-expressing MCF-7 cell lines were investigated by cytotoxicity test (XTT). P-glycoprotein reversing ability and MDR modulation effects of the extracts were determined by flow cytometry through Rhodamine 123 exclusion assay. Furthermore, 11 phenolic compounds in the extracts were characterized by HPLC. As a result of the cytotoxicity assay, IC50 values of the extracts for MCF-7/Vinc were between 1.08 and 1.80 mg/ mL, and IC50 values for MCF-7/Pac were found between 1.11 and 2.83 mg/mL. Strikingly, methanol extract of F. fomentarius and ethanol extract of T. anatolicum have potential value to become MDR reversing agents for drug-resistant breast cancer cells. © 2020 by Begell House.
Açıklama
Anahtar Kelimeler
Antitumor effect, Fomes fomentarius, MDR reversal, Medicinal mushrooms, Tricholoma anatolicum
Kaynak
International Journal of Medicinal Mushrooms
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
22
Sayı
2
