Sıçanlarda Deneysel Ercp Modelinde; Kontrast Madde Uygulama Hızının ve Kanal İçi Basınç Artışının Etkileri
Yükleniyor...
Tarih
2020 Mart
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmanın amacı ERCP işlemi sonrası akut pankreatit gelişiminde biliopankreatik kanaldaki basınç artışının ve opak madde verilme hızının etkilerini belirlemektir.
Gereç ve Yöntem: Çalışmamızda 24 adet rat 4 gruba ayrıldı. Grup 1 de sadece biliopankreatik kanal kanülasyonu yapıldı. Diğer gruplara biliopankreatik kanal içerisine farklı hızlarda opak madde infüzyonu uygulandı ve kanal içinde oluşan basınç ölçüldü.
Bulgular: Deney sonrası sıçanlar takibe alındı. 24 saat sonra akut pankreatit gelişimi ve şiddetinin değerlendirilmesi amacıyla sıçanlar
sakrifiye edilerek kan ve doku örnekleri alındı. Alınan kan örneğinden lökosit sayısı, amilaz, lipaz, üre, kreatinin, glukoz, AST, ALT,
ALP, LDH, kalsiyum, CRP, albümin, total protein, MDA, TAS, TOS değerleri çalışıldı. Pankreas dokuları akut pankreatit gelişimi
açısından histopatolojik olarak değerlendirildi.
Sonuç: Kanal içi hidrostatik basınç artışın akut pankreatit gelişmesine neden olmaktadır ve biliopankreatikkanal içerisindeki hidrostatik basınç arttıkça, pankreatitin şiddetinin de arttığı gözlenmiştir.
Objective: In this experimental study, we investigated the possible correlation between the increase in the hydrostatic pressure within the pancreatic duct caused by opaque substance given during ERCP procedure and the development of acute pancreatitis. Material and Methods: In our study, 24 rats were divided into 4 groups. The first of these groups was designated as the control group and only biliopancreatic duct cannulation was performed. In other groups, the opaque substance was infused into the biliopancreatic duct at various rates and the resulting intra-ductal pressure was measured. Results: After the experiment, the rats were followed up. 24 hours later, the rats were sacrificed, and blood and tissue samples were taken to evaluate the development and severity of acute pancreatitis. Leukocyte (WBC) count, amylase, lipase, urea, creatinine, glucose, AST, ALT, ALP, LDH, calcium, CRP, albumin, total protein, MDA, TAS and TOS were studied from the blood samples taken. The pancreatic tissues were histopathologically evaluated for the development of acute pancreatitis. Conclusion: it has been concluded that an increase in the intra-ductal hydrostatic pressure led to acute pancreatitis. As the hydrostatic pressure in the biliopancreatic duct increased, the severity of acute pancreatitis was observed to have increased
Objective: In this experimental study, we investigated the possible correlation between the increase in the hydrostatic pressure within the pancreatic duct caused by opaque substance given during ERCP procedure and the development of acute pancreatitis. Material and Methods: In our study, 24 rats were divided into 4 groups. The first of these groups was designated as the control group and only biliopancreatic duct cannulation was performed. In other groups, the opaque substance was infused into the biliopancreatic duct at various rates and the resulting intra-ductal pressure was measured. Results: After the experiment, the rats were followed up. 24 hours later, the rats were sacrificed, and blood and tissue samples were taken to evaluate the development and severity of acute pancreatitis. Leukocyte (WBC) count, amylase, lipase, urea, creatinine, glucose, AST, ALT, ALP, LDH, calcium, CRP, albumin, total protein, MDA, TAS and TOS were studied from the blood samples taken. The pancreatic tissues were histopathologically evaluated for the development of acute pancreatitis. Conclusion: it has been concluded that an increase in the intra-ductal hydrostatic pressure led to acute pancreatitis. As the hydrostatic pressure in the biliopancreatic duct increased, the severity of acute pancreatitis was observed to have increased
Açıklama
Anahtar Kelimeler
ERCP, akut pankreatit, ERCP sonrası akut pankreatit, kanal içi hidrostatik basınç artışı, acute pancreatitis, post-ERCP acute pancreatitis, intraductal hydrostatic pressure increase
Kaynak
Selçuk Genel Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
30
Sayı
1
Künye
Yormaz, S., Şahin, E., (2020). Sıçanlarda Deneysel Ercp Modelinde; Kontrast Madde Uygulama Hızının ve Kanal İçi Basınç Artışının Etkileri. Selçuk Genel Tıp Dergisi, 30(1), 58-65.
