Phytochemical profiling, in vitro biological properties and in silico studies on Caragana ambigua stocks (Fabaceae): A comprehensive approach

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dc.contributor.authorKhan, Saima
dc.contributor.authorNazir, Mamona
dc.contributor.authorRaiz, Naheed
dc.contributor.authorSaleem, Muhammad
dc.contributor.authorZengin, Gökhan
dc.contributor.authorFazal, Gazala
dc.contributor.authorSaleem, Hammad
dc.contributor.authorMukhtar, Mahreen
dc.contributor.authorTousif, Muhammad Imran
dc.contributor.authorTareen, Rasool Baksh
dc.contributor.authorAbdallah, Hassan Hadi
dc.contributor.authorMahomoodally, Fawzi Mohamed
dc.date.accessioned2020-03-26T20:19:09Z
dc.date.available2020-03-26T20:19:09Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractPlant from Caragana genus have interesting potential in folklore medicines and have been explored for various pharmacological activities. The aim of this study was to probe into the chemical and biological effects of different extracts of Caragana ambigua Stocks (Fabaceae). Total phenolic and total flavonoids contents were determined using standard spectrophotometric methods, whereas, the secondary metabolites composition was established by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) analysis. Antioxidant potential was estimated via 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), phosphomolybdenum and metal chelating assays and the enzyme (cholinesterases, alpha-amylase, alpha-glucosidase and tyrosinase) inhibition potential were also assessed. Moreover, in silico docking studies were performed to highlight possible interactions between three major secondary metabolites and the tested enzymes. The ethyl acetate extract exhibited higher phenolic (85.87 +/- 2.96 mg GAE/g) and flavonoid (66.45 +/- 0.37 mg RE/g) contents, which, we propose, are responsible for its higher radical scavenging, reducing power, total antioxidant capacity and tyrosinase inhibition. The n-hexane extract showed stronger anti-cholinesterase and anti-diabetic property. Similarly, UHPLC-MS profiling of methanol and ethyl acetate extracts identified the presence of flavonoids, phenolics, alkaloids, and coumarin derivatives. Three of the dominant compounds (isobergaptene, jujubasaponin IV and phellodensin D), were docked against all enzymes and their affinity were compared. Among these compounds, phellodensin D showed highest affinity towards all the studied enzymes. It is therefore, concluded that extracts from C. ambigua showed potent antioxidant and enzyme inhibition potential with potent bioactive molecules which could open new industrial applications.en_US
dc.identifier.citationKhan, S., Nazir, M., Raiz, N., Saleem, M., Zengin, G., Fazal, G., Saleem, H., Mukhtar, M., Tousif, M. I., Tareen, R. B., Abdallah, H. H., Mahomoodally, F. M. (2019). Phytochemical Profiling, in vitro Biological Properties and in Silico Studies on Caragana ambigua Stocks (Fabaceae): A Comprehensive Approach. Industrial Crops and Products, 131, 117-124.
dc.identifier.doi10.1016/j.indcrop.2019.01.044en_US
dc.identifier.endpage124en_US
dc.identifier.issn0926-6690en_US
dc.identifier.issn1872-633Xen_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage117en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.indcrop.2019.01.044
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38110
dc.identifier.volume131en_US
dc.identifier.wosWOS:000460991700015en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorZengin, Gökhan
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofINDUSTRIAL CROPS AND PRODUCTSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectCaragana ambiguaen_US
dc.subjectPhytochemicalsen_US
dc.subjectAntioxidanten_US
dc.subjectTyrosinaseen_US
dc.subjectMolecular dockingen_US
dc.titlePhytochemical profiling, in vitro biological properties and in silico studies on Caragana ambigua stocks (Fabaceae): A comprehensive approachen_US
dc.typeArticleen_US

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