Influence of lycopene and cysteamine on sperm and oxidative stress parameters during liquid storage of ram semen at 5 degrees C

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dc.contributor.authorPeker Akalin, Pinar
dc.contributor.authorBucak, Mustafa Numan
dc.contributor.authorGungor, Sukru
dc.contributor.authorBaspinar, Nuri
dc.contributor.authorCoyan, Kenan
dc.contributor.authorDursun, Sukru
dc.contributor.authorIli, Pinar
dc.date.accessioned2020-03-26T19:24:42Z
dc.date.available2020-03-26T19:24:42Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractEjaculates were collected from six Merino rams with the aid of an artificial vagina twice a week. The ejaculates containing spermatozoa with >80% forward progressive motility and concentrations higher than 2 x 10(6) spermatozoa/ml were pooled. The present study included two experiments. In experiment 1, each pooled ejaculate was divided into four equal aliquots and diluted (37 degrees C) with the Tris based extender, containing 0 (control), 0.5, 1 and 2 mM lycopene, at a final concentration of approximately 400 x 10(6) sperms/ml (single step dilution), In experiment 2, cysteamine at concentrations of 0 (control), 0.5,1 and 2 mM, was used as an additive in the extender, and the procedure explained above was applied for the division of aliquots and the dilution of semen. Diluted semen samples were kept in glass tubes and cooled from 37 degrees to 5 degrees C in a cold cabinet, and maintained at 5 degrees C. Sperm and oxidative stress parameters were evaluated after 0, 24, 48 and 72 h of storage at 5 degrees C. The extender supplemented with 0.5 mM lycopene resulted in higher mitochondrial activity rate (p<0.05) in comparison to the control group at 72 h of storage. Lycopene at 0.5 mM dose led to higher sperm motility rate (p<0.05) when compared to 2 mM lycopene group at 72 h of liquid storage. As regards oxidative stress parameters, only 2 mM lycopene increased total glutathione levels (p<0.05) at 0 h of storage. The extender supplemented with 1 mM cysteamine gave higher motility (p<0.05) at 48 h compared to control. As regards oxidative stress parameters, 1 and 2 mM cysteamine at 48 h and 1 mM cysteamine at 72 h increased total glutathione levels (p<0.05) compared to control groups. Cysteamine at 1 and 2 mM doses decreased lipid peroxidation (p<0.05) at 0 h of liquid storage compared to control. Our data suggest that lycopene at 0.5 and 2 mM and cysteamine at 1 and 2 mM doses can be added to Tris based extender for improving the ram sperm motility, viability, mitochondrial activity and oxidative stress parameters during the liquid storage. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112 O 845]en_US
dc.description.sponsorshipThe study was financed under the project supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No: 112 O 845).en_US
dc.identifier.doi10.1016/j.smallrumres.2016.03.017en_US
dc.identifier.endpage123en_US
dc.identifier.issn0921-4488en_US
dc.identifier.issn1879-0941en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage117en_US
dc.identifier.urihttps://dx.doi.org/10.1016/j.smallrumres.2016.03.017
dc.identifier.urihttps://hdl.handle.net/20.500.12395/33710
dc.identifier.volume137en_US
dc.identifier.wosWOS:000375504400019en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BVen_US
dc.relation.ispartofSMALL RUMINANT RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectFluorescent stainingen_US
dc.subjectLiquid storageen_US
dc.subjectLycopeneen_US
dc.subjectCysteamineen_US
dc.subjectRam spermen_US
dc.subjectOxidative stress parametersen_US
dc.titleInfluence of lycopene and cysteamine on sperm and oxidative stress parameters during liquid storage of ram semen at 5 degrees Cen_US
dc.typeArticleen_US

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