Assessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurements

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dc.contributor.authorAksoy, Emine
dc.contributor.authorAktan, Tahsin Murad
dc.contributor.authorDuman, Selcuk
dc.contributor.authorCuce, Gokhan
dc.date.accessioned2020-03-26T18:24:00Z
dc.date.available2020-03-26T18:24:00Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe aim was to examine the morphology of spermatozoa with different staining methods and aimed to find the better staining methods for morphology of spermatozoa in our study. Randomized 67 patients taken for the study who were admitted to Assisted Reproductive Techniques Unit. In the first part of the study, smears were stained with Hematoxylin Eosin (HE), Toluidin Blue (TB), Giemsa, Wright, ferrous Weigert haematoxylin stain, Orange G, eosin-aniline blue dye, Shorr Method, Papanicolau, Berg Method, Light Green stain, Acridine Orange (AO) and Janus Green dyes. In the second part of the study, smear preparations of 10 patients with normozoospermic were stained with HE, Toluidin Blue (TB), Shorr Method and Papanicolau. Four measurements were made including the middle piece, head length- head width and tail length for 200 spermatozoa with normal morphology. Comparisons were made between the stains that which showed a better morphology. Condensation assessment was not possible in smears stained with Shorr, Berg, Method, AO. Better assessment of condensation could be made in other stains. In the second part the smallest values belonged to of TB stain according to measurements of head of the spermatozoa. There was a significant difference at the head length with TB stain. Although measurements of Shorr and Papanicolau are close to each other and the largest values belonged to Papanicolau dye. It was concluded that measurement values in human sperm morphology could alter with the used staining method.en_US
dc.identifier.doi10.4067/S0717-95022012000400045en_US
dc.identifier.endpage1550en_US
dc.identifier.issn0717-9502en_US
dc.identifier.issn0717-9367en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1544en_US
dc.identifier.urihttps://dx.doi.org/10.4067/S0717-95022012000400045
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27768
dc.identifier.volume30en_US
dc.identifier.wosWOS:000319484200045en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSOC CHILENA ANATOMIAen_US
dc.relation.ispartofINTERNATIONAL JOURNAL OF MORPHOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectSpermatozoaen_US
dc.subjectMorphologyen_US
dc.subjectHistological stainsen_US
dc.titleAssessment of Spermatozoa Morphology under Light Microscopy with Different Histologic Stains and Comparison of Morphometric Measurementsen_US
dc.typeArticleen_US

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