Effects of Asphodeline lutea Compounds on Toxicity Models in Isolated Rat Microsomes and Hepatocytes

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dc.contributor.authorKondeva-Burdina, Magdalena
dc.contributor.authorSimeonova, Rumyana
dc.contributor.authorVitcheva, Vessela
dc.contributor.authorLazarova, Irina
dc.contributor.authorGevrenova, Reneta
dc.contributor.authorZheleva-Dimitrova, Dimitrina
dc.contributor.authorZengin, Gökhan
dc.date.accessioned2020-03-26T19:53:38Z
dc.date.available2020-03-26T19:53:38Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractBackground: In the current study, we evaluate the possible in vitro hepatoprotective and antioxidant activity of Asphodeline lutea (L.) Rchb. dry root extract (ALE), and isolated from the same extract 2-acetyl-1,8-dimethoxy-3-methylnaphthalene (1). The potential of the main root compounds, chrysophanol (2) and caffeic acid (3), was studied as well. A model of non-enzyme lipid peroxidation (LPO) in isolated liver microsomes was induced by iron-ascorbic acid (Fe2+/AA) mixture and assessed by the quantity of malondialdehyde (MDA) -an LPO product. The incubation of the microsomes with ALE (1 mg/ml) and 1-3 (100 mu g/ml) resulted in a significant decrease in MDA production, compared to the Fe2+/AA incubated samples with 23% (ALE), 61 % (1), 62% (3), while classical hepatoprotector silymarin decreased the parameter with 64 %. Methods: Studied compounds showed some toxicity in isolated rat hepatocytes discerned by increased LDH leakage and MDA quantity, decreased cell viability and reduced glutathione (GSH) levels compared to the control (non-treated hepatocytes). Results: The antioxidant and hepatoprotective potential of 1-3 was observed in vitro against carbon tetrachloride (CCl4)-induced toxicity, where they normalize all the examined parameters pertur-bated by CCl4 administration. The effects of 1 are lower than 3 and silymarin, but were better than those of 2. Conclusion: On the basis of these results, we discuss a bidirectional potential of the assayed parameters that might be explained with naphthalene transformation in cytochrom P450-dependent oxidation by CYP3A. The lack of metabolism and bioactivation of CCl4 could explain the cytoprotective effects of 1-3. The pro-oxidant effects of 1 and 2, in in vitro models, could be due to naphthalene and anthraquinone bioactivation pathways involving toxic metabolites.en_US
dc.description.sponsorshipMedical Science Council at the Medical University - Sofia, Bulgaria [13/2014]en_US
dc.description.sponsorshipThis work was supported by a grant 13/2014 from the Medical Science Council at the Medical University - Sofia, Bulgaria.en_US
dc.identifier.doi10.2174/1570180814666170306122707en_US
dc.identifier.endpage255en_US
dc.identifier.issn1570-1808en_US
dc.identifier.issn1875-628Xen_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage251en_US
dc.identifier.urihttps://dx.doi.org/10.2174/1570180814666170306122707
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36557
dc.identifier.volume15en_US
dc.identifier.wosWOS:000423791700006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherBENTHAM SCIENCE PUBL LTDen_US
dc.relation.ispartofLETTERS IN DRUG DESIGN & DISCOVERYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectMicrosomesen_US
dc.subjecthepatocytesen_US
dc.subjectmalondialdehydeen_US
dc.subjectAsphodeline lutea (L.) Rchb.en_US
dc.subjectanthraquinonesen_US
dc.subjectnaphthalenesen_US
dc.titleEffects of Asphodeline lutea Compounds on Toxicity Models in Isolated Rat Microsomes and Hepatocytesen_US
dc.typeArticleen_US

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