Immobilized copper-ion affinity adsorbent based on a cross-linked beta-cyclodextrin polymer for adsorption of Candida rugosa lipase

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dc.contributor.authorYılmaz, Elif
dc.contributor.authorSezgin, Mehmet
dc.contributor.authorYılmaz, Mustafa
dc.date.accessioned2020-03-26T17:38:54Z
dc.date.available2020-03-26T17:38:54Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractLipase from Candida rugosa was immobilized on a beta-cyclodextrin-based polymer by adsorption and subsequent cross-linking with epichlorohydrin (EP-CD). The ligand iminodiacetic acid (IDA) was then bonded with the cross-linked beta-cyclodextrin (EP-CD-IDA). This affinity adsorbent was further chelated with Cu2+ for the purpose of binding affinity and stability. The properties of the immobilized lipase were assayed and compared with those of the free enzyme. Results showed that 266 mu g protein with an activity of 17.85 U was bound per gram of matrix, giving 188% of the specific activity of the free enzyme and a total recovered activity of 79.7% under the optimum conditions. The pH and thermal stabilities of lipase were improved after immobilization on the beta-cyclodextrin-based polymer (EP-CD-IDA-Cu2+). In addition, experimental results indicated that the residual activity of the immobilized lipase was 50% after eight cycles of reuse.en_US
dc.description.sponsorshipScientific Research Foundation of Selcuk University, Konya, TurkeySelcuk University [08101024]en_US
dc.description.sponsorshipWe thank the Scientific Research Foundation of Selcuk University, Konya, Turkey (BAP Grant Number 08101024) for financial support of this work.en_US
dc.identifier.doi10.3109/10242420903242805en_US
dc.identifier.endpage366en_US
dc.identifier.issn1024-2422en_US
dc.identifier.issn1029-2446en_US
dc.identifier.issue05.06.2020en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage360en_US
dc.identifier.urihttps://dx.doi.org/10.3109/10242420903242805
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23602
dc.identifier.volume27en_US
dc.identifier.wosWOS:000272087700010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofBIOCATALYSIS AND BIOTRANSFORMATIONen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectLipase immobilizationen_US
dc.subjectCandida rugosa lipaseen_US
dc.subjectepichlorohydrinen_US
dc.subjectiminodiacetic aciden_US
dc.subjectbeta-cyclodextrinen_US
dc.titleImmobilized copper-ion affinity adsorbent based on a cross-linked beta-cyclodextrin polymer for adsorption of Candida rugosa lipaseen_US
dc.typeArticleen_US

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