Antimicrobial resistances of Escherichia coli isolated from Buteo rufinus
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Date
2018
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Volume Title
Publisher
Selçuk Üniversitesi Veterinerlik Fakültesi
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info:eu-repo/semantics/openAccess
Abstract
Amaç: Bu çalışmada, kızıl şahinlerden (Buteo rufinus) toplanan kloakal sıvap örneklerinin bakteriyolojik muayenesi ve izole edilen Escherichia coli (E. coli) suşlarının antibakteriyel dirençlerinin belirlenmesi amaçlandı. Gereç ve Yöntem: Çeşitli şikâyetlerle Afyon Kocatepe Üniversitesi Veteriner Fakültesi kliniklerine getirilen kızıl şahinlerden 24 kloakal sıvap örneği toplandı. Örnekler besi yerlerine ekilerek 37 °C de aerobik ve mikroaerofilik olarak inkübe edildi. Salmonella spp. varlığı ISO 6579 protokolüne göre yapıldı. Daha sonra izole edilen bakterilerin antibiyotik duyarlılıkları belirlendi. Bulgular: Örneklerden toplam 24 E. coli suşu izole edildi. Ayrıca, bir Enterococcus faecalis izolatı da izole edildi. E. coli izolatlarının tamamı florfenikol ve siprofoksasine karşı duyarlı olarak bulunurken diğer antibiyotiklere karşı değişen oranlarda dirençlilik tespit edildi. Öneri: Bu çalışma ile ülkemizdeki kızıl şahinlerden elde edilen kloakal sıvapların bakteriyolojik muayenesi ilk kez yapıldı. Ayrıca, antibiyotik kullanılmayan kızıl şahinlerden izole edilen E. coli izolatlarının farklı antibiyotiklere değişen oranlarda dirençli olması da anlamlı bulundu.
Aim: In this study, it was aimed to determine microbiological examination of cloacal swab specimens taken from Long-legged Buzzards (Buteo rufinus) and antibiotic resistance of isolated Escherichia coli (E. coli) strains. Materials and Methods: The cloacal swab specimens were obtained from 24 Long-legged Buzzards which were admitted to Afyon Kocatepe University Veterinary Faculty clinics. The samples were cultured on different media for various bacteria (Salmonella species, E. coli, Mycoplasma, Gram-positive cocci, etc.). The media were incubated at 37°C in aerobic and microaerophilic conditions. ISO 6579 protocol was applied for Salmonella. In addition, antibiotic susceptibilities of the isolated bacteria were determined. Results: Twenty-four E. coli were isolated from all cloacal swab samples. In addition, Enterococcus faecalis was isolated from one sample. While all E. coli isolates were susceptible to florfenicol and ciprofloxacin, other antibiotics were found to be susceptible at different rates. Conclusion: This study is important because it is the first microbiological examination of cloacal swaps of Long-legged Buzzards in this Country. In addition, it was significantly evaluated that E. coli strains were resistant to various antibiotics with different ratios while no antibiotics are used using antibiotics in Long-legged Buzzards
Aim: In this study, it was aimed to determine microbiological examination of cloacal swab specimens taken from Long-legged Buzzards (Buteo rufinus) and antibiotic resistance of isolated Escherichia coli (E. coli) strains. Materials and Methods: The cloacal swab specimens were obtained from 24 Long-legged Buzzards which were admitted to Afyon Kocatepe University Veterinary Faculty clinics. The samples were cultured on different media for various bacteria (Salmonella species, E. coli, Mycoplasma, Gram-positive cocci, etc.). The media were incubated at 37°C in aerobic and microaerophilic conditions. ISO 6579 protocol was applied for Salmonella. In addition, antibiotic susceptibilities of the isolated bacteria were determined. Results: Twenty-four E. coli were isolated from all cloacal swab samples. In addition, Enterococcus faecalis was isolated from one sample. While all E. coli isolates were susceptible to florfenicol and ciprofloxacin, other antibiotics were found to be susceptible at different rates. Conclusion: This study is important because it is the first microbiological examination of cloacal swaps of Long-legged Buzzards in this Country. In addition, it was significantly evaluated that E. coli strains were resistant to various antibiotics with different ratios while no antibiotics are used using antibiotics in Long-legged Buzzards
Description
Keywords
Kızıl şahin, kloakal sıvap, Escherichia coli, antimikrobiyal direnç, Long-legged Buzzards, cloacal swap, antimicrobial resistance
Journal or Series
Eurasian Journal of Veterinary Sciences
WoS Q Value
Scopus Q Value
Volume
34
Issue
3
Citation
Birdane M. F. , Aras Z., Gölen G. S., Yaprakci M. V., Hadimli H. H. (2018). Kızıl şahinlerden izole edilen Escherichia coli suşlarının
antimikrobiyal direnç profilleri. Eurasian Journal of Veterinary Sciences, 34 (3), 185-188 DOI: 10.15312/EurasianJVetSci.2018.199.
