Cranberry and Grape Juices Affect Tight Junction Function and Structural Integrity of Rotavirus-Infected Monkey Kidney Epithelial Cell Monolayers

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dc.contributor.authorLipson, Steven M.
dc.contributor.authorGordon, Robert E.
dc.contributor.authorOzen, Fatma S.
dc.contributor.authorKarthikeyan, Laina
dc.contributor.authorKirov, Nicolas
dc.contributor.authorStotzky, Guenther
dc.date.accessioned2020-03-26T18:14:03Z
dc.date.available2020-03-26T18:14:03Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractCranberry juice (CJ) and grape juice (GJ) from Vaccinium macrocarpon and Vitis labrusca, respectively, and purified proanthocyanidins (PACs) from these species are recognized to possess antiviral activity. The effects of CJ and GJ on tight junction (TJ) structure and function among rotavirus-infected monkey kidney epithelial cells (MA-104) in monolayer cultures were evaluated. Antiviral activity by cranberry PACs of rotavirus in cell-free suspension was investigated by a rotavirus antigen [i.e., viral capsid protein 6 (VP6)] capture enzyme-linked immunosorbent assay (ELISA) and by transmission electron microscopy (TEM). MA-104 monolayers were treated with CJ, GJ, or cranberry juice cocktail (CJC) drink before inoculation with rotavirus. TJ function and structural integrity were measured by changes in transepithelial electrical resistance (TEER) and by reduction of signal intensity of the TJ alpha-claudin 1 by immunofluorescence. The inhibitory activity of CJ and GJ on viral RNA synthesis, as a function of viral concentration, was determined by reverse transcription polymerase chain reaction (rtPCR). After 4 days, virus-infected monolayers pretreated with GJ (Concord and Niagara GJs) had TEER readings similar to uninfected controls. CJ and CJC also had a significant protective effect (P < 0.05) on TJ function, but to a lesser extent than GJ. Disorganization of TJ integrity commenced at 24- to 36-h post-viral inoculation, but this effect was reduced by pretreatment with CJ or GP of monolayer cultures. TEM showed aggregation of rotavirus by cranberry PACs. The destruction of rotavirus capsid proteins VP6, in cell-free suspension was inversely related to the concentration of cranberry PACs (C-PAC). Loss of rotavirus RNA by CJ or GJ was inversely related to viral infectivity titers. CJ, GJ, or PAC-associated antiviral activity has been linked to modifications in cellular physiologic events and to physical factors (e.g., PAC-mediated viral aggregation) that probably compromise viral infectivity. Multiple cell physiological and physical events must be considered when determining the mechanisms associated with the antiviral (i.e., rotavirus) activity of CJ, GJ, and PACs.en_US
dc.description.sponsorshipCranberry Institute; Wisconsin and Canadian Cranberry Growers Association; Welch Foods, Inc.; Metropolitan Association of College and University Biologists (MACUB); St. Francis College Faculty Research and Developmenten_US
dc.description.sponsorshipThis work was supported, in part, by the Cranberry Institute, the Wisconsin and Canadian Cranberry Growers Association, Welch Foods, Inc., the Metropolitan Association of College and University Biologists (MACUB), and St. Francis College Faculty Research and Development. We appreciate the technical assistance of M. Preet and thank H. P. Lipson for proofreading the manuscript.en_US
dc.identifier.doi10.1007/s12560-011-9055-8en_US
dc.identifier.endpage54en_US
dc.identifier.issn1867-0334en_US
dc.identifier.issn1867-0342en_US
dc.identifier.issue1en_US
dc.identifier.pmid35255646en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage46en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s12560-011-9055-8
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26289
dc.identifier.volume3en_US
dc.identifier.wosWOS:000288554000005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofFOOD AND ENVIRONMENTAL VIROLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectRotavirus infectivityen_US
dc.subjectProanthocyanidinsen_US
dc.subjectCell function/structureen_US
dc.subjectTight junctionsen_US
dc.subjectElectron microscopyen_US
dc.titleCranberry and Grape Juices Affect Tight Junction Function and Structural Integrity of Rotavirus-Infected Monkey Kidney Epithelial Cell Monolayersen_US
dc.typeArticleen_US

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