Determination of protein degradability of alfalfa hay via buffer or protease

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dc.contributor.authorInal, Fatma
dc.contributor.authorTamkoc, Ahmet
dc.contributor.authorAlatas, Mustafa Selcuk
dc.contributor.authorKahraman, Oguzhan
dc.contributor.authorOzbilgin, Abdullah
dc.contributor.authorCoskun, Behic
dc.date.accessioned2020-03-26T19:53:21Z
dc.date.available2020-03-26T19:53:21Z
dc.date.issued2018
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis study was conducted to determine the effect of different vegetative periods on protein fractions of alfalfa hay and to compare two different methods for estimation of its protein degradability. In this study, 44 alfalfa hay samples cut in late vegetative, late bud, early bloom and late bloom were used. Crude protein decreased with advancing maturity (p<.05), but neutral detergent insoluble nitrogen and acid detergent insoluble nitrogen were similar. Protein degradability of the samples was estimated using Streptomyces griseus protease or borate-phosphate buffer, kinetic model, as described in Cornell Net Carbohydrate Protein System (CNCPS). Alfalfa hay samples were subjected to proteolysis for 30h with 0.115 U/mL or 0.230 U/mL of the protease at pH 6.8. There was a moderate correlation (0.66, 0.72) between enzymatic and buffer method estimates with protein degradation. As vegetation progresses, rapidly degradable fraction (A) decreased (p < .05) and undegradable fraction (C) increased (p < .05), while potentially degradable fraction (B) was unchanged. Rumen degradable protein content decreased (p < .05) with advancing maturity. The enzyme/duration limits should be standardised and investigated whether the protease enzyme alone is sufficient for all forages.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordination UnitSelcuk University [14401115]en_US
dc.description.sponsorshipThis project was funded by Selcuk University Scientific Research Projects Coordination Unit [Project number: 14401115].en_US
dc.identifier.doi10.1080/1828051X.2017.1364986en_US
dc.identifier.endpage358en_US
dc.identifier.issn1594-4077en_US
dc.identifier.issn1828-051Xen_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage353en_US
dc.identifier.urihttps://dx.doi.org/10.1080/1828051X.2017.1364986
dc.identifier.urihttps://hdl.handle.net/20.500.12395/36480
dc.identifier.volume17en_US
dc.identifier.wosWOS:000424424400012en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTAYLOR & FRANCIS LTDen_US
dc.relation.ispartofITALIAN JOURNAL OF ANIMAL SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectAlfalfa hayen_US
dc.subjectvegetationen_US
dc.subjectprotein fractionsen_US
dc.subjectenzymeen_US
dc.titleDetermination of protein degradability of alfalfa hay via buffer or proteaseen_US
dc.typeArticleen_US

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