Optimization of DNA isolation for RAPD-PCR analysis of selected (Echinaceae Purpurea L. Moench) medicinal plants of conservation concern from Turkey

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dc.contributor.authorVural, Hasibe Cingilli
dc.contributor.authorDageri, Asli
dc.date.accessioned2020-03-26T17:39:29Z
dc.date.available2020-03-26T17:39:29Z
dc.date.issued2009
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractGenetic analysis of plants relies on high yields of pure DNA samples Here we present the optimization of DNA isolation and PCR conditions for RAPD analysis of selected medicinal plants of conservation concern from Turkey containing high levels of polysaccharides, polyphenols and secondary metabolites The method involves a modified CTAB extraction employing polyvinyl pyrrolidone (PVP) while grinding successive long term chloroform isoamylalcohol extractions, EZ1 nucleic acid isolation protocols The yield of DNA ranged from 1 2 mu g/mu l per gram of the leaf tissue and the purity (ratio) was between 1 7 1 8 indicating minimal levels of contaminating metabolites EZ1 nucleic acid isolation technique is ideal for isolation of DNA from different plant species and the DNA isolated was used for randomly amplified polymorphic DNA (RAPD) analysis RAPD protocol was optimized based on the use of higher concentration of MgCl(2) (3 mM) lower concentrations of primer (0 5 mu M) and Taq polymerase (0 2 units) 50 ng of template DNA and an annealing temperature of 37 degrees C, resulted optimal amplification Reproducible amplifiable products were observed in all PCR reactions Thus the results indicate that the optimized protocol for DNA isolation and PCR was amenable to plant species belonging to different genera which is suitable for further work on diversity analysis Furthermore here we used suitable DNA isolation protocol for RAPD analysis to study the genetic variation in the future in Echinaceae sp grown in Turkeyen_US
dc.description.sponsorshipSelcuk University Archeometry laboratorySelcuk Universityen_US
dc.description.sponsorshipWe would like to thank Mr A Ahmet Tirpan for his technical assistance This study was partially supported by Selcuk University Archeometry laboratoryen_US
dc.identifier.endpage1087en_US
dc.identifier.issn1996-0875en_US
dc.identifier.issue12en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1084en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/23729
dc.identifier.volume3en_US
dc.identifier.wosWOS:000281784800016en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherACADEMIC JOURNALSen_US
dc.relation.ispartofJOURNAL OF MEDICINAL PLANTS RESEARCHen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectEchinaceae purpureaen_US
dc.subjectrandom amplified polymorphicen_US
dc.subjectDNAen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectmedicinal planten_US
dc.titleOptimization of DNA isolation for RAPD-PCR analysis of selected (Echinaceae Purpurea L. Moench) medicinal plants of conservation concern from Turkeyen_US
dc.typeArticleen_US

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