The application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessions

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dc.contributor.authorArslan, Emine
dc.contributor.authorTamkoc, Ahmet
dc.date.accessioned2020-03-26T18:16:33Z
dc.date.available2020-03-26T18:16:33Z
dc.date.issued2011
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThe present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat (ISSR) markers. ISSR amplification was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identification. A total of 401 bands, 363 of which were polymorphic (90.52%), were generated using selected 20 primers. Genetic similarity between accessions belonging to Poa species was estimated using Nei's genetic similarity and UPGMA (unweighted pair group method with arithmetic) cluster analysis was used to construct a dendrogram. UBC836 and M6 primers were observed to be the most suitable for distinguishing between species. Because interspecies differences are especially important in plant breeding, M5 and UBC812 primers were ideal in distinction of P. trivialis and P angustifolia accessions, respectively. Primer UBC856 was determined to be ideal in discrimination both intraspecies and interspecies. The ISSR fingerprinting technique used was confirmed to be a reproducible and sensitive tool for the identification of Poa accessions and for determination of the correct starting material for plant breeding.en_US
dc.identifier.doi10.3906/biy-0912-31en_US
dc.identifier.endpage423en_US
dc.identifier.issn1300-0152en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage415en_US
dc.identifier.urihttps://dx.doi.org/10.3906/biy-0912-31
dc.identifier.urihttps://hdl.handle.net/20.500.12395/26888
dc.identifier.volume35en_US
dc.identifier.wosWOS:000294698400004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEYen_US
dc.relation.ispartofTURKISH JOURNAL OF BIOLOGYen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectPoa angustifoliaen_US
dc.subjectPoa trivialisen_US
dc.subjectintersimple sequence repeat (ISSR)en_US
dc.subjectgenetic relationshipen_US
dc.subjectgenetic diversityen_US
dc.titleThe application of ISSR-PCR to determine the genetic relationship and genetic diversity between narrow leaved bluegrass (Poa angustifolia) and rough bluegrass (Poa trivialis) accessionsen_US
dc.typeArticleen_US

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