Boğaz Kültürleri?nden I?zole Edi?len Beta-hemoli?ti?k Streptokoklarin I?denti?fi?kasyonu, Ti?plendi?ri?lmesi? Ve Anti?bi?yoti?k Ve Eri?tromi?si?n Di?renç Profi?lleri?ni?n Beli?rlenmesi?
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Dosyalar
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Fen Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
A grubu beta hemolitik streptokoklar (AGBHS) sıklıkla üst solunum yolu enfeksiyonlarından izole edilen ajanlardan biridir. Streptokok enfeksiyonları dünyada, öncelikle streptokok farenjit olmak üzere, doktor muayenesi, ilaç tedavisi ve iş günü kayıplarını kapsayan çok fazla miktarda maddi kayıplara neden olmaktadır. Çalışmamızda AGBHS'ların API 20 Strep sistemi ile identifikasyonu, patojen türlerin sayı ve oranları ve kullanılan antibiyotiklere dirençlerinin belirlenmesi amaçlanmıştır. Konya şehir merkezindeki çeşitli hastanelerde, akut tonsillofarenjit ön tanısı konulan hastalardan boğaz kültürü örnekleri alındı. Örnekler peptonlu suda bir saat ön inkübasyona tabi tutuldu. Ardından % 5 koyun kanlı Mueller-Hinton Agar’a (MHA) ekildi. Suşların grupları lateks aglütinasyon testi (SLIDEX ® Strepto Plus, Biomerieux) ile belirlendi. 373 örnek içerisinde Basitrasin’e duyarlı, Trimetoprim-sulfometaksazol'e dirençli, katalaz negatif, gram pozitif toplam 96 suş beta-hemolitik streptokok (AGBHS) olarak tanımlandı. Ayrıca API 20 Strep sistemi ile 96 adet AGBHS suşu tür seviyesinde sınıflandırıldı. CLSI önerileri doğrultusunda disk difüzyon yöntemi ile AGBHS suşlarının kullanılan antibiyotiklere karşı direnç ve duyarlılıkları belirlendi. Suşların antibiyotik direnç profilleri, çoklu antibiyotik direnç indeksleri ve eritromisin direnci tespit edildi. Çalışmadan elde edilen verilere göre, örneklerin % 25.73'ü AGBHS olarak tanımlandı. 96 suşun 13 farklı tür içerisinde dağılım gösterdiği görüldü. En baskın türlerin sırasıyla; Streptococcus acidominimus (34 suş), Streptococcus pyogenes (23 suş), Streptococcus oralis (9 suş), Streptococcus mitis 1 (7 suş) ve Streptococcus agalactiae (7 suş) olduğu belirlendi. Suşlar en fazla direnci tetrasikline (14 suş, 14.5%), takiben ampisilin ve eritromisine (13 suş, % 13.5); klaritromisine (10 suş, % 10.4); klindamisine (4 suş, % 4.1) gösterdi. AGBHS'lar birbirinden farklı 16 direnç profili ortaya koydu. Çoklu antibiyotik direnç indeksine göre 8 suş 0.2 değerinden büyük bir indeks gösterdi. Sonuç olarak, ülkemizde AGBHS suşlarının patojen profilleri belirlendi ve eritromisin direncinin ülkemizde önceden yapılan çalışmaların verileri ile kıyaslandığında yüksek olduğu ortaya kondu. Bu oranın şimdilik sorun olmadığı fakat aralıklı olarak direnç seviyelerinin ülke genelinde izlenmesinin faydalı olacağı kanaatine varıldı
Group A beta-haemolytic streptococci (GABHS) is one of the agents which are frequently isolated from upper respiratory tract infections. Streptococcal infections, primarily including streptococcal pharyngitis, cause too much financial loss every year in the form of doctors’ visits, medication, and lost workdays in the world. In our study, identification of GABHSs by API 20 Strep system and determination of number of pathogenic species and ratios, and resistance to antibiotics used were aimed. Throat culture samples were taken from the patients pre-diagnosed with acute tonsillopharyngitis in various hospitals in the city center of Konya. The samples were kept in the peptone water as one hour as for preincubation. Then they were cultured on 5% sheep blood Mueller-Hinton Agar (MHA). Groups of the strains were determined by latex agglutination test (SLIDEX ® Strepto Plus, Biomerieux). Total of 96 strains which were bacitracin sensitive, trimethoprim-sulfomethaxazole resistant, catalase negative, gram positive were identified as beta-hemolytic streptococci (GABHS) in 373 samples. In addition, 96 GABHS strains were classified at the species level by API 20 Strep system. Resistance and susceptibilities of GABHS strains against antibiotics used were determined by disc diffusion method conducted in accordance with CLSI recommendations. The antibiotic resistance profiles, multiple antibiotic resistance indexes and erythromycin resistance of the strains were detected. According to the data obtained from study, 25.73% of the samples were defined as the GABHS. It was seen that 96 strains separated into 13 different species. It was determined that the dominant species were Streptococcus acidominimus (34 strain), Streptococcus pyogenes (23 strain), Streptococcus oralis (9 strain), Streptococcus mitis 1 (7 strain) ve Streptococcus agalactiae (7 strain), respectively. The strains showed the greatest resistance to tetracycline (14 strain, 14.5%), followed by ampicillin and erythromycine (13 strains, 13.5%), clarithromycin (10 strains, 10.4%), clindamycin (4 strains, 4.1%). GABHSs revealed 16 different types of resistance profiles. According to multiple antibiotic resistance indexes, 8 strains showed an index greater than 0.2 values. Consequently, pathogen profiles of GABHS strains were determined and it was revealed that erythromycin resistance was higher when compared to data of previous studies conducted in our country. It was concluded that this rate is not a problem now, but monitoring of the resistance levels intermittently throughout the country would be useful.
Group A beta-haemolytic streptococci (GABHS) is one of the agents which are frequently isolated from upper respiratory tract infections. Streptococcal infections, primarily including streptococcal pharyngitis, cause too much financial loss every year in the form of doctors’ visits, medication, and lost workdays in the world. In our study, identification of GABHSs by API 20 Strep system and determination of number of pathogenic species and ratios, and resistance to antibiotics used were aimed. Throat culture samples were taken from the patients pre-diagnosed with acute tonsillopharyngitis in various hospitals in the city center of Konya. The samples were kept in the peptone water as one hour as for preincubation. Then they were cultured on 5% sheep blood Mueller-Hinton Agar (MHA). Groups of the strains were determined by latex agglutination test (SLIDEX ® Strepto Plus, Biomerieux). Total of 96 strains which were bacitracin sensitive, trimethoprim-sulfomethaxazole resistant, catalase negative, gram positive were identified as beta-hemolytic streptococci (GABHS) in 373 samples. In addition, 96 GABHS strains were classified at the species level by API 20 Strep system. Resistance and susceptibilities of GABHS strains against antibiotics used were determined by disc diffusion method conducted in accordance with CLSI recommendations. The antibiotic resistance profiles, multiple antibiotic resistance indexes and erythromycin resistance of the strains were detected. According to the data obtained from study, 25.73% of the samples were defined as the GABHS. It was seen that 96 strains separated into 13 different species. It was determined that the dominant species were Streptococcus acidominimus (34 strain), Streptococcus pyogenes (23 strain), Streptococcus oralis (9 strain), Streptococcus mitis 1 (7 strain) ve Streptococcus agalactiae (7 strain), respectively. The strains showed the greatest resistance to tetracycline (14 strain, 14.5%), followed by ampicillin and erythromycine (13 strains, 13.5%), clarithromycin (10 strains, 10.4%), clindamycin (4 strains, 4.1%). GABHSs revealed 16 different types of resistance profiles. According to multiple antibiotic resistance indexes, 8 strains showed an index greater than 0.2 values. Consequently, pathogen profiles of GABHS strains were determined and it was revealed that erythromycin resistance was higher when compared to data of previous studies conducted in our country. It was concluded that this rate is not a problem now, but monitoring of the resistance levels intermittently throughout the country would be useful.
Açıklama
Anahtar Kelimeler
API 20 strep, A grubu beta hemolitik streptokoklar, Antibiyotik direnç profili, İdentifikasyon, Eritromisin direnci, Group a beta hemolytic streptococci, Antibiotic resistance profile, Identification, Erythromycin resistance
Kaynak
Selçuk Üniversitesi Fen Fakültesi Fen Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
39
Künye
Uysal, A., Durak, Y., Akın, D. (2014). Boğaz kültürleri̇nden izole edi̇len beta-hemoli̇ti̇k streptokoklarin identi̇fi̇kasyonu, ti̇plendi̇ri̇lmesi̇ ve anti̇bi̇yoti̇k ve eri̇tromi̇si̇n di̇renç profi̇lleri̇ni̇n beli̇rlenmesi̇. Selçuk Üniversitesi Fen Fakültesi Fen Dergisi, (39), 10-20.
