Nerium oleander distilatının antimikrobiyal etkisinin araştırılması
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Dosyalar
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Mevcut araştırmanın amacı Nerium oleander distilatının antimikrobial etkisini araştırmaktır. Gereç ve Yöntem: Toplanan Nerium oleander bitkisi tanımlandı ve onaylandı. Nerium oleander yıkandı ve doğrandı, sonrasında yeterli oranda su ilave edildi. Karışım distilastorde distile edildi. Bitki-distile su karışımı buharlaştırılmaya başlandıktan sonra buhar kısmı temiz cam kaplara ayrıştırıldı. Elde edilen Nerium oleander distilatı küçük cam şişeler içinde liyofilize edildi ve kuru materyal toplandı. Liyofilize kuru materyal 5 mg/mL olacak şekilde distile suda çözdürüldü ve Escherichia coli, Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Metisilin Rezistans Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumonia, Listeria monocytogenes, Pseudomonas aeruginosa ve Candida ssp.'ya karşı antimikrobiyal etkisi çukur agardifüzyon metodu ile araştırıldı. Bulgular: Nerium oleander distilatının incelenen hiçbir mikroorganizmaya karşı etkili olmadığı belirlendi. Öneri: Nerium oleander distilatının kimyasal ekstraklarının aksine antimikrobiyal etkisinin olmadığı ifade edilebilir.
Aim: The aim of the current study was to determine the antimicrobial effect of Nerium oleander distillate. Material and Method: Collected Nerium oleander plant was identified and authenticated. Nerium oleander was washed and chopped, later sufficient distilled water was added. The mixture was distillated by distillatory device. After the plantdistillated water mixture was started to evaporate, vapor was separated to other clean glass containers. Obtained Nerium oleander distillate was lyophilized in small glass bottle, and dry material was collected. Lyophilized dry material was dissolved at concentration of 5 mg/mL with distillated water, and its antimicrobial effect was investigated against to Escherichia coli, Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Methicillin Resistant Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumonia, Listeria monocytogenes, Pseudomonas aeruginosa and Candida ssp. by agar well-diffusion method. Result: Nerium oleander distillate did no show any antimicrobial effect against to tested microorganism. Conclusion: It may be stated that Nerium oleander distillate has no antimicrobial effect contrary to its chemical extracts.
Aim: The aim of the current study was to determine the antimicrobial effect of Nerium oleander distillate. Material and Method: Collected Nerium oleander plant was identified and authenticated. Nerium oleander was washed and chopped, later sufficient distilled water was added. The mixture was distillated by distillatory device. After the plantdistillated water mixture was started to evaporate, vapor was separated to other clean glass containers. Obtained Nerium oleander distillate was lyophilized in small glass bottle, and dry material was collected. Lyophilized dry material was dissolved at concentration of 5 mg/mL with distillated water, and its antimicrobial effect was investigated against to Escherichia coli, Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Methicillin Resistant Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumonia, Listeria monocytogenes, Pseudomonas aeruginosa and Candida ssp. by agar well-diffusion method. Result: Nerium oleander distillate did no show any antimicrobial effect against to tested microorganism. Conclusion: It may be stated that Nerium oleander distillate has no antimicrobial effect contrary to its chemical extracts.
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
29
Sayı
3
Künye
Dik, B., Sayın, Z., Çorum, O. (2013). Nerium Oleander Distilatının Antimikrobiyal Etkisinin Araştırılması. Eurasian Journal of Veterinary Sciences, 29(3), 150-152.
