Testis torsiyonundaki iskemi-reperfüzyon hasarını önlemede melatonin ve steroidin tek doz ve yedi günlük tedavilerinin etkileri
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Tarih
2005
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Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Amaç: Çalışmamızda, torsiyon ve detorsiyon sonrası testiste oluşan iskemi-reperfüzyon (İ-R) hasarını önlemek için uygulanan melatonin ve steroidin tek doz ve 7 günlük tedavilerinin etkilerini ve her iki tedavi şeklinden hangisinin daha etkili olduğunu ortaya koymayı amaçladık. Gereç ve Yöntemler: Çalışmaya alınan sıçanlar 10’arlı 8 gruba ayrıldı. Sham grupları hariç tüm gruplarda sol testisler 6 saat süre ile 720o torsiyone edildi. Detorsiyondan 30 dk. önce melatonin gruplarına 17 mg/kg melatonin, steroid gruplarına ise 1 mg/kg metilprednizolon verildi. Yedi doz melatonin ve 7 doz steroid gruplarında 7 gün süre ile tedaviye devam edildi. Sham-1, torsiyon-detorsiyon-1, tek doz melatonin ve tek doz steroid gruplarında detorsiyondan 6 saat sonra, diğer gruplarda ise 7 gün sonra sol orşiektomi yapıldı. İ-R hasarını değerlendirmek amacıyla testis dokusunda malondialdehit (MDA) seviyelerine ve histopatolojik inceleme yapılarak Johnsen skorlarına bakıldı. Bulgular: Tek doz melatonin ve tek doz steroid gruplarının MDA seviyeleri torsiyon-detorsiyon-1 grubuna göre daha düşük olduğu (p 0.05) ancak Johnsen skorlarının aynı olduğu (p 0.05) belirlendi. Yedi doz melatonin ve 7 doz steroid gruplarında ise MDA seviyelerinin torsiyon-detorsiyon-2 grubundan daha düşük olduğu (p 0.01), Johnsen skorlarının da daha yüksek olduğu (p 0.01) tespit edildi. Sonuç: Testis torsiyonunda İ-R hasarını önlemek için detorsiyondan önce tek doz olarak verilen melatonin ve steroid biyokimyasal olarak etkili gibi gözükmesine rağmen histopatolojik olarak hiç bir etkisi yoktur. Ancak 7 gün süre ile uygulanan melatonin ve steroid İ-R hasarını tam olarak önlemektedir.
Objective: The aim of the study is to determine the effects of melatonin and steroid treatments which were injected as one-dose and one-dose for seven days to prevent ischemia-reperfusion injury occurred in testicle after torsion and detorsion and which of these treatments is more effective. Material and Methods: Eighty prepubertal male Sprague-Dawley rats were devided into 8 groups each containing 10 rats. In all groups, except sham groups, torsion was created by rotating the left testicles 720o in a clockwise direction for 6 hours. In melatonin groups 17 mg/kg melatonin and in steroid groups 1 mg/kg methylprednesolone were injected before 30 minutes from detorsion. In seven dose steroid and melatonin groups melatonin and methylprednesolone were injected once a day for 7 days. In Sham-1, torsion-detorsion-1, one-dose melatonin and, one-dose steroid groups left orchiectomies were created after 6 hours of detorsion. In other group, left orchiectomies were performed 7 days later. After performing histopathologic examination we determined MDA levels and Johnsen&#8217;s scores of testicular tissue to identify ischemia-reperfusion injury. Results: MDA levels in one-dose melatonin and steroid groups were significantly less compared with torsion-detorsion-1 group (p< 0.05). Johnsen&#8217;s scores were the same as in one-dose melatonin, steroid and torsion-detorsion-1 groups (p> 0.05). It was identified that MDA levels in seven dose melatonin and seven dose steroid groups were significantly less compared with torsion-detorsion-2 group (p< 0.01) and Johnsen&#8217;s scores were significantly higher compared with torsion-detorsion-2 group (p< 0.01). Conclusion: The treatments of one-dose melatonin and steroid are effective against the I-R damage biochemically, but not histopathologically. Whereas, melatonin and steroid, which were injected once a day for 7 days, prevent ischemia-reperfusion injury completely
Objective: The aim of the study is to determine the effects of melatonin and steroid treatments which were injected as one-dose and one-dose for seven days to prevent ischemia-reperfusion injury occurred in testicle after torsion and detorsion and which of these treatments is more effective. Material and Methods: Eighty prepubertal male Sprague-Dawley rats were devided into 8 groups each containing 10 rats. In all groups, except sham groups, torsion was created by rotating the left testicles 720o in a clockwise direction for 6 hours. In melatonin groups 17 mg/kg melatonin and in steroid groups 1 mg/kg methylprednesolone were injected before 30 minutes from detorsion. In seven dose steroid and melatonin groups melatonin and methylprednesolone were injected once a day for 7 days. In Sham-1, torsion-detorsion-1, one-dose melatonin and, one-dose steroid groups left orchiectomies were created after 6 hours of detorsion. In other group, left orchiectomies were performed 7 days later. After performing histopathologic examination we determined MDA levels and Johnsen&#8217;s scores of testicular tissue to identify ischemia-reperfusion injury. Results: MDA levels in one-dose melatonin and steroid groups were significantly less compared with torsion-detorsion-1 group (p< 0.05). Johnsen&#8217;s scores were the same as in one-dose melatonin, steroid and torsion-detorsion-1 groups (p> 0.05). It was identified that MDA levels in seven dose melatonin and seven dose steroid groups were significantly less compared with torsion-detorsion-2 group (p< 0.01) and Johnsen&#8217;s scores were significantly higher compared with torsion-detorsion-2 group (p< 0.01). Conclusion: The treatments of one-dose melatonin and steroid are effective against the I-R damage biochemically, but not histopathologically. Whereas, melatonin and steroid, which were injected once a day for 7 days, prevent ischemia-reperfusion injury completely
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Türkiye Klinikleri Tıp Bilimleri Dergisi
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25
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4
