Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods
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Dosyalar
Tarih
Mart 2022
Yazarlar
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Cilt Başlığı
Yayıncı
Selçuk Üniversitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada, Türkiye’de tüketilen yüzgeçli balıklarda ve karideslerde Vibrio spp., Aeromonas spp. ve Listeria spp. varlığının ve elde edilen izolatların antibiyotik direnç profilinin disk difüzyon yöntemi kullanılarak belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Araştırmada, farklı zamanlarda toplanan 300 adet deniz ürünü materyal olarak kullanılmıştır. Klasik kültürel yöntemle izolasyonun ardından, izolatları cins düzeyinde doğrulamak ve patojen türleri tanımlamak için klasik PCR yapılmıştır. Bulgular: Yüzgeçli balık ve karides örnekleri Vibrio spp., Aeromonas spp. ve Listeria spp. ile sırasıyla %19,4 (33/170), %14,7 (25/170), %4,1 (7/170) and %13,8 (18/130), %13,1 (17/130), %6,2 (8/130) oranında kontamine bulunmuştur. Vibrio spp. izolatlarının 29 (%9,7)'u ve 9 (%3,0)’u sırasıyla V. parahaemolyticus ve V. cholerae olarak tanımlanmıştır. Aeromonas spp. izolatlarının 30 (%10)'u A. hydrophila olarak tespit edilmiştir. Örneklerin hiçbirinde L. monocytogenes ve V. vulnificus saptanmamıştır. V. parahaemolyticus, V. cholerae, A. hydrophila ve Listeria spp. izolatlarının antibiyotik direnç profili sırası ile streptomisin (%71,4), teikoplanin (%71,4), ampisilin (%87,5), teikoplanin (%75); streptomisin (%80), ampisilin (%75), sefiksim (%75), penisilin (%75), sulfametoksazol/trimetoprim (%100), tetrasiklin (%75); eritromisin (%93,8), vankomisin (%81,2), amoksasilin/ klavulanik asit (%78,6), ampisilin (%85,7), sefalotin (%92,9), penisilin G (%92,9); ve sefalotin (%37,5-%42,9), eritromisin (%37,5-%42,9), penisilin G (%37,5-%42,9), tetrasiklin (%37,5-%42,9) olarak tespit edilmiştir. Öneri: Sonuç olarak, Türkiye'de tüketilen balık ve karideslerin patojen Vibrio ve Aeromonas türleri ile kontamine olabilecekleri ve antibiyotik dirençli izolatların halk sağlığı açısından risk oluşturabileceği düşünülmektedir.
Aim: In this study, it was aimed to determine the presence of Vibrio spp., Aeromonas spp., and Listeria spp. in finned fish and shrimps consumed in Turkey and the antibiotic resistance profile of the isolates using disc diffusion method. Materials and Methods: In the research, 300 seafoods obtained at different times were used as material. Following isolation by classical cultural method, classical PCR was performed to confirm the isolates at genus level and to identify at species level for pathogenic species. Results: Finfish and shrimp samples were contaminated with Vibrio spp., Aeromonas spp. and Listeria spp. with the rate of 19.4% (33/170), 14.7% (25/170), 4.1% (7/170) and 13.8% (18/130), 13.1% (17/130), 6.2% (8/130), respectively. Twenty-nine (9.7%) and 9 (3.0%) of the Vibrio spp. isolates were identified as V. parahaemolyticus and V. cholerae, respectively. Thirty (10%) of the Aeromonas spp. isolates were detected as A. hydrophila. L. monocytogenes and V. vulnificus was not detected in any of samples. Antibiotic resistance profile of the V. parahaemolyticus, V. cholerae, A. hydrophila and Listeria spp. isolates was streptomycin (71.4%), teicoplanin (71.4%), ampicillin (87.5%), teicoplanin (75%); streptomycin (80%), ampicillin (75%), cefixime (75%), penicillin (75%), sulfamethoxazole/trimethoprim (100%), tetracycline (75%); erythromycin (93.8%), vancomycin (81.2%), amoxacillin/clavulanic acid (78.6%), ampicillin (85.7%), cephalothin (92.9%), penicillin G (92.9%); and cephalothin (37.5%-42.9%), erythromycin (37.5%-42.9%), penicillin G (37.5%-42.9%), tetracycline (37.5%-42.9%), respectively. Conclusion: As a result, it is thought that fish and shrimp consumed in Turkey may be contaminated with pathogenic Vibrio and Aeromonas species, and antibiotic-resistant isolates may pose a risk to public health.
Aim: In this study, it was aimed to determine the presence of Vibrio spp., Aeromonas spp., and Listeria spp. in finned fish and shrimps consumed in Turkey and the antibiotic resistance profile of the isolates using disc diffusion method. Materials and Methods: In the research, 300 seafoods obtained at different times were used as material. Following isolation by classical cultural method, classical PCR was performed to confirm the isolates at genus level and to identify at species level for pathogenic species. Results: Finfish and shrimp samples were contaminated with Vibrio spp., Aeromonas spp. and Listeria spp. with the rate of 19.4% (33/170), 14.7% (25/170), 4.1% (7/170) and 13.8% (18/130), 13.1% (17/130), 6.2% (8/130), respectively. Twenty-nine (9.7%) and 9 (3.0%) of the Vibrio spp. isolates were identified as V. parahaemolyticus and V. cholerae, respectively. Thirty (10%) of the Aeromonas spp. isolates were detected as A. hydrophila. L. monocytogenes and V. vulnificus was not detected in any of samples. Antibiotic resistance profile of the V. parahaemolyticus, V. cholerae, A. hydrophila and Listeria spp. isolates was streptomycin (71.4%), teicoplanin (71.4%), ampicillin (87.5%), teicoplanin (75%); streptomycin (80%), ampicillin (75%), cefixime (75%), penicillin (75%), sulfamethoxazole/trimethoprim (100%), tetracycline (75%); erythromycin (93.8%), vancomycin (81.2%), amoxacillin/clavulanic acid (78.6%), ampicillin (85.7%), cephalothin (92.9%), penicillin G (92.9%); and cephalothin (37.5%-42.9%), erythromycin (37.5%-42.9%), penicillin G (37.5%-42.9%), tetracycline (37.5%-42.9%), respectively. Conclusion: As a result, it is thought that fish and shrimp consumed in Turkey may be contaminated with pathogenic Vibrio and Aeromonas species, and antibiotic-resistant isolates may pose a risk to public health.
Açıklama
Anahtar Kelimeler
Aeromonas spp, antibiyotik direnç, Listeria spp, deniz ürünleri, Vibrio spp, Aeromonas spp, antibiotic resistance, Listeria spp, seafoods, Vibrio spp
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
38
Sayı
1
Künye
Telli, N., Telli, A. E., Biçer, Y., Turkal, G., Kahraman, H. A., Doğruer, Y. (2022). Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. Eurasian Journal of Veterinary Sciences 38 ,(1), 7-16.
