RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey
Küçük Resim Yok
Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
UNIV CATOLICA DE VALPARAISO
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Cannabis sativa L. is a multiple-use plant. However, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 29 different locations of Turkey, were used. Interests were to identify the genetic relatedness of the seized samples and to partition molecular variance between and within populations. Randomly Amplified Polymorphic DNAs were employed for analysis based on single plant material and bulked samples of them. Data were analysed via cluster and principal coordinate analyses (PCoA). Analysis of molecular variance (AMOVA) was performed to obtain variations between and within populations. Cannabis accessions were basically separated into two main groups by PCoA and cluster analyses according to geographical regions. One of them was made up of Cannabis plants, which were seized from mostly western part of Turkey (group 1). The other one was made up of Cannabis plants that were seized from mostly eastern part of Turkey (group 2). It is found that 20.23% of the genetic variation is due to differences between accessions groups while 79.77% of the genetic variation is due to between accessions within accessions groups. Compared to group 1, group 2 showed more variation.
Açıklama
Anahtar Kelimeler
AMOVA, cluster analysis, individualization of seized marijuana, principle coordinate analysis, randomly amplified polymorphic DNA, UPGMA
Kaynak
ELECTRONIC JOURNAL OF BIOTECHNOLOGY
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
12
Sayı
1
