Lentinula edodes (Berk.) Pegler 1976’ in Misel Gelişmesine Humik Maddeler ve Giberellik Asidin Etkisinin Araştırılması
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Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada Lentinula edodes’in misel gelişimi üzerine hümik maddeler ve giberellik asidin (GA3) etkisi araştırılmıştır. Malt ekstrakt agar (MEA) ve patates dekstroz agar (PDA) besi yeri olarak kullanılmış olup, bu besiyer ortamlarına, hümik madde (HM) ve giberellik asidin (GA3) belirli dozları ilave edilerek otoklavda 121°C’de 15 dk steril edilmiştir. Pastör fırınında steril edilmiş petrilere steril güvenlik kabini içerisinde besi yerleri dökülmüştür. Besi yerleri katılaştıktan sonra aynı kabin içerisinde misel ihtiva eden besi yeri aktarımı yapılmıştır. Bu işlemden sonra petriler etüve alınmıştır. Belli aralıklarla miselyum gelişmeleri alansal olarak çizilmiştir. Ön çalışma olarak besiyerlerine %0, %1, %3, %5, %7, %9 oranlarında hümik madde ve giberellik asidin (GA3) ilavesi yapılmış ve bu oranlarda yapılan uygulama sonuçlarına göre, çalışma denemelerinin %0, %0.5, %1, %1.5 oranlarında yapılmasına karar verilmiştir. Bu çalışmada MEA ve PDA besiyerlerinin hazırlanması esnasında %0, %0.5, %1 ve %1.5 oranında hümik madde ve giberellik asidin (GA3) eklenerek petrilere aktarılmıştır. Lentinula edodes’in misel gelişim miktarları ve yoğunlukları incelenmiştir. Denemeler 3 tekrarlı olarak yapılmıştır. Deneme sonuçlarına göre yapılan varyans analizlerine göre ortalamalar arasındaki farklar istatistiki olarak önemli bulunmuştur (P<0.01). %0, %0.5, %1 ve %1.5 oranında hümik madde ve giberellik asit (GA3) uygulamaları yönünden değerlendirme yapıldığında gelişim miktarı ve katkı maddeleri oranları arasında yüksek bir korelasyon tespit edilememiş olup, MEA besi ortamında 0.5 ve 1.5’ lik hümik madde uygulamasında nispeten yüksek gelişim olduğu görülmüştür (R² = 0.370).
Abstract In this study, the effect of humic substances and gibberellic acid (GA3) on mycelial development of Lentinula edodes was investigated. Malt extract agar (MEA) and potato dextrose agar (PDA) were used as feedstock in the study and malt extract agar and potato dextrose agar were sterilized by autoclaving at 121°C for 15 min with the addition of specific doses of agaric material. In the Pasteur oven, nutrients were poured into sterile petri dishes in the sterile safety cabinet. After the feedstock sites solidified, the transfer of the media containing the mycelium was carried out in the same cabin. After this process, the petri dishes were taken to the incubation oven. At certain intervals mycelial developments were drawn spatially. As a preliminary study, 0%, 1%, 3%, 5%, 7% and 9% of humic substance and gibberellic acid (GA3) were added to feedstock and according to the results of the applications made at these rates, it has been decided to make the working trials at 0%, 0.5%, 1%, 1.5%. In this study, malt extract agar and potato dextrose agar supplemented with 0%, 0.5%, 1% and 1.5% of humic substance and gibberellic acid (GA3) were added to petri dishes. Micelle growth amounts and densities of Lentinula edodes were investigated. The experiments were done in 3 replicates. According to the analysis of variance with trial results the differences between the means were found to be statistically significant (P <0.01). When %0, 0.5%, 1% and 1.5% of humic substance and gibberellic acid (GA3) applications were evaluated, no high correlation was found between the amount of growth and additives and only a relatively high improvement was observed in the application of 0.5% and 1.5% humic substance in MEA feedstock (R² = 0.370).
Abstract In this study, the effect of humic substances and gibberellic acid (GA3) on mycelial development of Lentinula edodes was investigated. Malt extract agar (MEA) and potato dextrose agar (PDA) were used as feedstock in the study and malt extract agar and potato dextrose agar were sterilized by autoclaving at 121°C for 15 min with the addition of specific doses of agaric material. In the Pasteur oven, nutrients were poured into sterile petri dishes in the sterile safety cabinet. After the feedstock sites solidified, the transfer of the media containing the mycelium was carried out in the same cabin. After this process, the petri dishes were taken to the incubation oven. At certain intervals mycelial developments were drawn spatially. As a preliminary study, 0%, 1%, 3%, 5%, 7% and 9% of humic substance and gibberellic acid (GA3) were added to feedstock and according to the results of the applications made at these rates, it has been decided to make the working trials at 0%, 0.5%, 1%, 1.5%. In this study, malt extract agar and potato dextrose agar supplemented with 0%, 0.5%, 1% and 1.5% of humic substance and gibberellic acid (GA3) were added to petri dishes. Micelle growth amounts and densities of Lentinula edodes were investigated. The experiments were done in 3 replicates. According to the analysis of variance with trial results the differences between the means were found to be statistically significant (P <0.01). When %0, 0.5%, 1% and 1.5% of humic substance and gibberellic acid (GA3) applications were evaluated, no high correlation was found between the amount of growth and additives and only a relatively high improvement was observed in the application of 0.5% and 1.5% humic substance in MEA feedstock (R² = 0.370).
Açıklama
Anahtar Kelimeler
Lentinula edodes, Hümik Maddeler, Giberellik asit (GA3), Misel Gelişmesi, MEA, PDA, Humic substances, Gibberellic acid (GA3), Mycelium Growth
Kaynak
Mantar Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
11
Sayı
1
Künye
Kayahan, F., Kaşık, Gıyasettin, Kayahan, N., (2020). Lentinula edodes (Berk.) Pegler 1976’ in Misel Gelişmesine Humik Maddeler ve Giberellik Asidin Etkisinin Araştırılması. Mantar Dergisi, 11, (1), 40-49. Doi: 10.30708.mantar.610869
