The Effect of Oestradiol-17 Beta on the Motility, Viability and the Acrosomal Status of Bull Sperm
Yükleniyor...
Dosyalar
Tarih
2010
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SOUTH AFRICAN JOURNAL OF ANIMAL SCIENCES
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to test the effect of oestradio1-17 beta (E(2)) on the motility, viability and the acrosomal status of bull sperm in vitro. Pooled semen from Holstein bulls were incubated in the presence of 2, 4, and 8 mu g E(2)/mL for 24 h. Semen was also incubated in media without E(2). During the incubation, the number of motile, viable sperm and the number of sperm possessing lost/damaged acrosomes, cytoplasmic droplets and coiled tails were counted at 0, 4, 18 and 24 h of incubation. Addition of 2 mu g E(2)/mL at 18 h of incubation increased the total motility over the control. The number of forward progressing sperm was increased by the supplementation of 2 and 8 mu g E(2)/mL over the control group at the 4 h incubation. Lower doses of E(2) (2 and 4 mu g/mL) did not affect viability of sperm, but a high dose of E(2) (8 mu g/mL) caused reductions in viability at 4 and 24 h of incubation. The number of sperm cells with lost acrosomes was significantly high in control group at 24 h of incubation. The number of sperm cells possessing proximal and distal cytoplasmic droplets and the number of sperm cells bearing coiled tails were not altered by any of the treatments. A small dose of E(2) (2 mu g/mL) had a beneficial effect on the motility and acrosome integrity of bull sperm in vitro. Higher dose of E(2) (8 mu g/mL) had a detrimental effect on viability.
Açıklama
Anahtar Kelimeler
Bovine Males, Estradiol, Acrosome, Motility, Spermatozoa, In Vitro Incubation
Kaynak
South African Journal of Animal Science
WoS Q Değeri
Q3
Scopus Q Değeri
Cilt
40
Sayı
1
Künye
Çiftçi, H. B., Zülkadir, U., (2010). The Effect of Oestradiol-17 Beta on the Motility, Viability and the Acrosomal Status of Bull Sperm. South African Journal of Animal Science, 40(1), 6-13.
