Enzyme immobilization in biosensor constructions: self-assembled monolayers of calixarenes containing thiols

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dc.contributor.authorDemirkol, Dilek Odacı
dc.contributor.authorYıldız, Hüseyin Bekir
dc.contributor.authorSayın, Serkan
dc.contributor.authorYılmaz, Mustafa
dc.date.accessioned2020-03-26T18:50:31Z
dc.date.available2020-03-26T18:50:31Z
dc.date.issued2014
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractHerein, an amperometric glucose oxidase (GOx) biosensor is presented using calixarenes as an immobilization matrix of the biomolecule. Firstly, thiol-containing calixarenes (Calix-SH) were synthesized, then self-assembled monolayers (SAMs) of Calix-SH on a gold surface were formed and hydroxyl groups of Calix-SH were activated using 1,1'-carbonyldiimidazole (CDI) chemistry. To test the usability of Calix-SH modified surfaces as a biosensor, glucose oxidase was used as a model biological component. After optimization of preparation and working conditions, our results indicate that the Calix-SH/GOx biosensor has a linear range in the range 0.1-1.0 mM (LOD: 0.015 mM) for glucose with a 25 s response time. Finally, the application of the biosensor was examined to detect glucose in real samples. The glucose amounts were calculated as 19.460 +/- 0.521 and 31.647 +/- 2.125 mM in coke and fizzy drink (with orange), respectively. To confirm the reliability of the Calix-SH/GOx biosensor, the calculated glucose concentrations which were analyzed by the Calix-SH/GOx biosensor were compared to conventional spectrophotometric glucose kits. The glucose amounts in coke and fizzy drink were calculated as 18.509 +/- 0.732 mM and 31.579 +/- 4.466 mM, respectively.en_US
dc.description.sponsorshipEuropean Union through the COST Action [CM1202]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113T022]en_US
dc.description.sponsorshipAuthors would like to thank the European Union through the COST Action CM1202 "Supramolecular photocatalytic water splitting (PERSPECT-H<INF>2</INF>O)" and the Scientific and Technological Research Council of Turkey (TUBITAK Grant Numbers 113T022) for the financial support of this research.en_US
dc.identifier.doi10.1039/c3ra47642aen_US
dc.identifier.endpage19907en_US
dc.identifier.issn2046-2069en_US
dc.identifier.issue38en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage19900en_US
dc.identifier.urihttps://dx.doi.org/10.1039/c3ra47642a
dc.identifier.urihttps://hdl.handle.net/20.500.12395/30826
dc.identifier.volume4en_US
dc.identifier.wosWOS:000335922900048en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherROYAL SOC CHEMISTRYen_US
dc.relation.ispartofRSC ADVANCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.titleEnzyme immobilization in biosensor constructions: self-assembled monolayers of calixarenes containing thiolsen_US
dc.typeArticleen_US

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