Quantitative analysis of various virulance genes (PVL, LukED, ?-hemoliz) by real-time PCR of Staphylococcus aureus strains isolated from bovine mastitis
Küçük Resim Yok
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
University of Punjab (new Campus)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In 98 Staphylococcus aureus (S. aureus) strains isolated from bovine mastitis, presence/absence of virulence genes such as Panton-Valentine leukocidin (PVL), ?-hemolysis and LukED and quantitative analysis were conducted by Real-Time PCR. According to Real-Time PCR PVL, ?-hemolysis and LukED genes of 98 S. aureus strains were found as 70.40%, 90.81% and 66.32%, respectively. As a result of the quantitative analysis of virulence genes according to strains, they are grouped into 3 groups as 40<-10,000, 10,000-100,000 and 100,000< IU/ml considering viral gene amount. The amount of PVL gene among these groups was determined as 27.55%, 9.18% and 32.65% respectively, and ?-hemolysis as 48.97%, 39.79% and 2.04% and LukED as 45.91%, 14.28% and 6.12%. As a result, in S. aureus strains isolated from bovine mastitis the prevalence of these virulence genes were higher. Therefore it is deduced that PVL, ?-hemolysis and LukED may be important virulence factors in the pathogenesis of S. aureus. Copyright 2016 Zoological Society of Pakistan.
Açıklama
Anahtar Kelimeler
LukED, PVL, Real-Time PCR, Staphylococcus aureus mastitis, ?-hemolysis
Kaynak
Pakistan Journal of Zoology
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
48
Sayı
6
