Quantitative analysis of various virulance genes (PVL, LukED, ?-hemoliz) by real-time PCR of Staphylococcus aureus strains isolated from bovine mastitis

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dc.contributor.authorArslan E.
dc.contributor.authorLadikli S.
dc.contributor.authorArslan U.
dc.date.accessioned2020-03-26T19:32:03Z
dc.date.available2020-03-26T19:32:03Z
dc.date.issued2016
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractIn 98 Staphylococcus aureus (S. aureus) strains isolated from bovine mastitis, presence/absence of virulence genes such as Panton-Valentine leukocidin (PVL), ?-hemolysis and LukED and quantitative analysis were conducted by Real-Time PCR. According to Real-Time PCR PVL, ?-hemolysis and LukED genes of 98 S. aureus strains were found as 70.40%, 90.81% and 66.32%, respectively. As a result of the quantitative analysis of virulence genes according to strains, they are grouped into 3 groups as 40<-10,000, 10,000-100,000 and 100,000< IU/ml considering viral gene amount. The amount of PVL gene among these groups was determined as 27.55%, 9.18% and 32.65% respectively, and ?-hemolysis as 48.97%, 39.79% and 2.04% and LukED as 45.91%, 14.28% and 6.12%. As a result, in S. aureus strains isolated from bovine mastitis the prevalence of these virulence genes were higher. Therefore it is deduced that PVL, ?-hemolysis and LukED may be important virulence factors in the pathogenesis of S. aureus. Copyright 2016 Zoological Society of Pakistan.en_US
dc.identifier.endpage1632en_US
dc.identifier.issn0030-9923en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage1627en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/34308
dc.identifier.volume48en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniversity of Punjab (new Campus)en_US
dc.relation.ispartofPakistan Journal of Zoologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectLukEDen_US
dc.subjectPVLen_US
dc.subjectReal-Time PCRen_US
dc.subjectStaphylococcus aureus mastitisen_US
dc.subject?-hemolysisen_US
dc.titleQuantitative analysis of various virulance genes (PVL, LukED, ?-hemoliz) by real-time PCR of Staphylococcus aureus strains isolated from bovine mastitisen_US
dc.typeArticleen_US

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