Transmissible venereal tümörlü dişi köpeklerde otojen aşı uygulaması
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Dosyalar
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Selçuk Ünivesitesi Veterinerlik Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Yapılan bu çalışmada transmissible venereal tümör (TVT) tedavisinde otojen aşı uygulamalarının başarısının belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmanın hayvan materyalini 8 adet TVT’li dişi köpek oluşturdu. Köpeklere uygulanan otojen aşı bovine papillomatozis aşısı esas alınarak yapıldı. Yaklaşık 24 g tümör dokusuna 48 mL tris buffer (pH: 7.5) solüsyonu eklenerek otoklavize edilmiş steril kum yardımıyla soğuk şartlarda (+4 0 C) 15-20 dakika havan içerisinde ezilerek parçalandı. Karışımın yarısı (36 mL) alınıp tris buffer ile 60- 65 mL’ye tamamlandı ve %0.5 oranında formalin katılıp 10 mL’lik 6 kısma ayrıldı. Hazırlanan aşı 1 hafta ara ile 4 kez (SC) uygulandı. Köpekler 4. uygulamanın ardından 3 aylık takibe alındı ve tümörün gerilemesi yönünden değerlendirmeler yapıldı. Bulgular: Köpeklerde otojen aşı uygulaması öncesinde serosanguinöz veya hemorajik genital akıntı tespit edildi. Vagina ve vulvada bulunan kitleler karnabahar biçiminde ve dokunulduğunda kolayca kanayan bir yapıda gözlendi. Otojen aşı uygulamaları sonrasında köpeklerin dış genital organlarında bulunan kitlelerden gelen serosanguinöz veya hemorajik genital akıntının kesilmediği ve kitlenin frajilitesinde bir değişiklik olmadığı tespit edildi. Otojen aşı uygulaması yapılan hayvanlarda, uygulanan 4 doz aşı sonrasında vagina ve vulvada bulunan kitlelerde herhangi bir gerileme ve iyileşmenin olmadığı belirlendi. Öneriler: TVT oluşumunda viral bir ajanın etken olabileceğine inanılmaktadır. Orijini bovine papilloma virüs olan sığırların papillomatozisinin tedavisinde başarılı sonuçlar alınan otojen aşı uygulama tekniğinin köpeklerde TVT tedavisinde etkili olamayabileceği kanaatine varıldı.
Aim: The aim of the study was to determine the success of autogenous vaccine application in treatment of TVT. Materials and Methods: The study was conducted on 8 bitches with TVT. . The autogenous vaccine applied on the dogs was maddened the based on the bovine papillomatosis vaccine. Approximately 24 g of tumor tissue and 48 mL Tris buffer (pH 7.5) solution were crushed in cold conditions (+4 0 C) during 15-20 minutes in mortar disintegrated with autoclaved sterile sand. Half of the mixture (36 mL) was completed with tris buffer to 60-65 mL. 0.5 percent formalin was added this mixture and mixture was portioned to 6 each has 10 mL. The prepared vaccine was administered 4 times (SC) with an interval of 1 week. Dogs were followed up 3 months after 4th application and evaluations were performed for tumor regression. Results: Serosanguineous or hemorrhagic genital discharge was determined in dogs prior to application of autogenous vaccine. The lesions in external genital organs are like cauliflower, rubescent, fresh-looking and easily bleed on touched. After administration of the autogenous vaccine, serosanguineous or hemorrhagic genital discharge from the masses on the external genital organs of dogs continued, and there were no changes in the mass fragility. After the application of autogenous vaccines at four times, there was no any decline and improvement in the masses on the vagina and vulva. Conclusions: It is believed that a viral agent may contribute to the etiology of TVT. Prepared autogenous vaccine technique used in the treatment of bovine papillomatosis derived from bovine papilloma virus may not be suitable in the treatment of bitches with TVT.
Aim: The aim of the study was to determine the success of autogenous vaccine application in treatment of TVT. Materials and Methods: The study was conducted on 8 bitches with TVT. . The autogenous vaccine applied on the dogs was maddened the based on the bovine papillomatosis vaccine. Approximately 24 g of tumor tissue and 48 mL Tris buffer (pH 7.5) solution were crushed in cold conditions (+4 0 C) during 15-20 minutes in mortar disintegrated with autoclaved sterile sand. Half of the mixture (36 mL) was completed with tris buffer to 60-65 mL. 0.5 percent formalin was added this mixture and mixture was portioned to 6 each has 10 mL. The prepared vaccine was administered 4 times (SC) with an interval of 1 week. Dogs were followed up 3 months after 4th application and evaluations were performed for tumor regression. Results: Serosanguineous or hemorrhagic genital discharge was determined in dogs prior to application of autogenous vaccine. The lesions in external genital organs are like cauliflower, rubescent, fresh-looking and easily bleed on touched. After administration of the autogenous vaccine, serosanguineous or hemorrhagic genital discharge from the masses on the external genital organs of dogs continued, and there were no changes in the mass fragility. After the application of autogenous vaccines at four times, there was no any decline and improvement in the masses on the vagina and vulva. Conclusions: It is believed that a viral agent may contribute to the etiology of TVT. Prepared autogenous vaccine technique used in the treatment of bovine papillomatosis derived from bovine papilloma virus may not be suitable in the treatment of bitches with TVT.
Açıklama
Anahtar Kelimeler
Transmissible venereal tümör, otojen aşı, Transmissible venereal tumor, autogenous vaccine
Kaynak
Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
28
Sayı
4
Künye
Çizmeci, S. Ü., Köse, A. M., Aydın, İ., Dinç, D. A., Maden, M., Köse, S. İ., (2012). Transmissible venereal tümörlü dişi köpeklerde otojen aşı uygulaması. Eurasian Journal of Veterinary Sciences, 28 (4). 220-223.
