DETERMINATION OF GENETIC RELATIONSHIP IN TURKISH CHICKPEA (CICER ARIETINUM L.) GENOTYPES USING SSR MOLECULAR MARKERS AND CAPILLARY ELECTROPHORESIS

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dc.contributor.authorAtalay, E.
dc.contributor.authorBabaoglu, M.
dc.date.accessioned2020-03-26T18:24:43Z
dc.date.available2020-03-26T18:24:43Z
dc.date.issued2012
dc.departmentSelçuk Üniversitesien_US
dc.description.abstractThis study aimed to determinate the genetic relationship among 29 chickpea (Cicer arietinum L.) genotypes in Turkey using SSR markers. A total of 13 SSR primers were used for PCR amplifications, but the study was conducted with the data obtained from 10 SSR primers that yielded good results. PCR products were separated by the Beckman Coulter CEQ-8000 genetic analysis system with capillary electrophoresis and peak values of each primer were scored as presence (1) or absence (0) for all genotype. The dendrogram was constructed based on the genetic similarity coefficient using the UPGMA (unweighted pair group with arithmetic average) method with the computer software NTSYS-pc. Also, polymorphism rates, heterozygosity and polymorphic informaiton content were calculated using statistical formula. A total of 134 alleles were obtained from 10 primers and 122 of them were turned out to be polymorphic and the allele numbers obtained with SSR primers varied between 4 and 28. Heterozygosity also ranged between 0.23 and 0.42. Polymorphic information contents (PIC) varied between 0.70 and 0.89. Genetic similarity coefficients changed between 0.45 and 0.87. Chickpea genotypes identified among the very high level of similarity to that obtained from the dendogram branches intertwined. As a result, Turkish chickpea genotypes can be expressed in a very narrow genetic base.en_US
dc.description.sponsorshipSelcuk University Scientific Research FundSelcuk Universityen_US
dc.description.sponsorshipThis study is part of a PhD thesis by Emine Atalay and was supported by the Selcuk University Scientific Research Fund.en_US
dc.identifier.endpage375en_US
dc.identifier.issn1018-7081en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage369en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12395/27895
dc.identifier.volume22en_US
dc.identifier.wosWOS:000305681200022en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPAKISTAN AGRICULTURAL SCIENTISTS FORUMen_US
dc.relation.ispartofJOURNAL OF ANIMAL AND PLANT SCIENCESen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectChickpeaen_US
dc.subjectCicer arietinum L.en_US
dc.subjectSSRen_US
dc.subjectcapillary electrophoresisen_US
dc.subjectgenetic relationshipen_US
dc.titleDETERMINATION OF GENETIC RELATIONSHIP IN TURKISH CHICKPEA (CICER ARIETINUM L.) GENOTYPES USING SSR MOLECULAR MARKERS AND CAPILLARY ELECTROPHORESISen_US
dc.typeArticleen_US

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