Neonatal ishalli buzağılarda serum immunoglobulinlerin glutaraldehit koagulasyon testi ile değerlendirilmesi
Yükleniyor...
Dosyalar
Tarih
2000
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, neonatal buzağılarda ishalin pasif immun transferi ile ilgisi ve bunu belirlemede glutaraldehid (GA) koagulasyon testinin güvenirliliğinin, bir kantitatif test olan single radial immunodiffüzyon (SRID) testi kullanarak ortaya konulması amaçlanmıştır. Bu araştırmanın materyalini, 20 sağlıklı ve 30 ishalli olmak üzere toplam 50 adet neonatal buzağı oluşturdu, immunglobulin G seviyeleri, kantitatif olarak single radial immunodiffüzyon testi ile; semikantitatif olarakta glutaraldehid koagulasyon testi ile ölçüldü. Neonatal ishalli buzağılarda 30 dakikada pıhtılaşmayan serum örneklerinde IgG konsantrasyonları 250-400 mg/dl iken, 12-28. dakikada yarı pıhtılaşma (jelöz) gösteren serum örneklerinde ise IgG konsantrasyonları 580-800 mg/dl idi. Sağlıklı neonatal buzağılarda 1-5 dakikada pıhtılaşan serum örneklerinde IgG konsantrasyonları 2400-5800 mg/dl iken, 6-7 dakikada pıhtılaşan serum örneklerinde IgG konsantrasyonları 1600-1700 mg/dl idi. Sonuç olarak negatif ve jelöz glutaraldehid test sonucu veren buzağılarda yetersiz pasif kolostral transfere pozitif glutaraldehid test sonucu veren buzağılarda yeterli pasif kolostral transfer gözlenebileceği söylenebilir
The aim of this study, in neonatal calves, to carry out the relationship between diarrhea and passive co-lostral transfer and the safety of GA test using SRID (Single Radial Immunodiffussion) test for its determination. In this study, healty 20 and diarrheic 30, totally 50 calves were used as a material. Serum immunglobulin levels were me-asured by GA test as semiquantitative and by SRID test as kantitative. In the some diarrheic calves IgG levels were 250-400 mg/dl and their serurn incougulated in 30 minutes. In the meantime, IgG levels of other diarrhoeic calves were 580-800 mg/dl and their serum semisolid (jelous) at between 12-28 minutes. In the healty calves, serum samples with IgG level of 2400-5800 mg/dl coagulated in 1-5 minutes and serum samples with IgG level of 1600 mg/ dl coagulated in 6 to 7 minutes. As a result, it could be said that diarrhea is connected with passive colostral transfer, and calves with negative and jelous GA test have failure of passive colostral transfer and calves with positive GA test have adequate passive colostral transfer.
The aim of this study, in neonatal calves, to carry out the relationship between diarrhea and passive co-lostral transfer and the safety of GA test using SRID (Single Radial Immunodiffussion) test for its determination. In this study, healty 20 and diarrheic 30, totally 50 calves were used as a material. Serum immunglobulin levels were me-asured by GA test as semiquantitative and by SRID test as kantitative. In the some diarrheic calves IgG levels were 250-400 mg/dl and their serurn incougulated in 30 minutes. In the meantime, IgG levels of other diarrhoeic calves were 580-800 mg/dl and their serum semisolid (jelous) at between 12-28 minutes. In the healty calves, serum samples with IgG level of 2400-5800 mg/dl coagulated in 1-5 minutes and serum samples with IgG level of 1600 mg/ dl coagulated in 6 to 7 minutes. As a result, it could be said that diarrhea is connected with passive colostral transfer, and calves with negative and jelous GA test have failure of passive colostral transfer and calves with positive GA test have adequate passive colostral transfer.
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Veteriner Bilimleri Dergisi . Eurasian Journal of Veterinary Sciences
WoS Q Değeri
Scopus Q Değeri
Cilt
16
Sayı
1
Künye
Şen, İ., Başoğlu, A., Ok, M., Birdane, F. M., Güzelbektaş, H., Civelek, T. (2000). Neonatal İshalli Buzağılarda Serum İmmunoglobulinlerin Glutaraldehit Koagulasyon Testi ile Değerlendirilmesi. Veteriner Bilimleri Dergisi . Eurasian Journal of Veterinary Sciences, 16(1), 143-146.
